Biological sciences
Exploring Bacillus subtilis Isolated from Soil as a Promising Alternative for L-Asparaginase Enzyme Production

Seyed Mohammad Haji Mirghasemi; Hamid Reza Pordeli; Leila Fozouni; Mina Komshikamar; fatemeh komshikamar; Mansoor Rubin

Articles in Press, Accepted Manuscript, Available Online from 23 September 2023

https://doi.org/10.22034/hbb.2023.409710.1103

Abstract
  A high L-ASNase enzyme production by Bacillus subtilis can hold significant promise as an alternative bacterial source to meet anti-cancer therapies demand. In this study, eight bacterial isolates were obtained from the 35 soil samples of Golestan province, Iran. Samples were collected from different ...  Read More

Biological sciences
A review of similarity of KLF2, MSX1 and SOX9 genes expression in zebrafish and human

Mehdi Ahmadifar; Reihaneh Balali; sadaf reihani; zeinab jarban

Volume 7, Issue 2 , September 2023, , Pages 18-34

https://doi.org/10.22034/HBB.2023.15

Abstract
  In recent studies, the focus on zebrafish, as a suitable laboratory model for studying vertebrate gene function, has increased dramatically. We evaluated the similarity between the three genes KLF2, MSX1 and SOX9 in humans and zebrafish. The KLF2 gene plays a role in heart and immunity of humans and ...  Read More

Biological sciences
Preconditioning with IFN-γ and LPS improves the immunomodulatory potential of bone marrow-derived clonal mesenchymal stem cells

Mehdi Soleymani-Goloujeh; Faezeh Shekari; Seyedeh-Nafiseh Hassani; Ensiyeh Hajizadeh-Saffar

Volume 7, Issue 2 , September 2023, , Pages 35-51

https://doi.org/10.22034/HBB.2023.16

Abstract
  Mesenchymal Stem Cells (MSCs) are one of the promising cellular therapies for immune-related diseases. Preconditioning of MSCs increases their efficacy and overcomes several hurdles in MSC-based therapies. It reduces tissue inflammation and improves cell survival, consequently enhancing therapeutic outcomes. ...  Read More

Biological sciences
Genotyping of KELL blood group in multiethnic populations of Quetta, Pakistan

Asifa Bibi; Nabeela Tariq; Hamida Ali; Zil-e-Huma Butt; Tasleem Kausar; Maqsood Maryam

Volume 7, Issue 2 , September 2023, , Pages 66-81

https://doi.org/10.22034/HBB.2023.18

Abstract
  KEL1 (Kell or K) is highly immunogenic with limited distribution while KEL2 (Cellano or k) antigen is more prevalent. The antigenic diversity in the Kell gene is a result of Single Nucleotide Polymorphisms (SNPs). Blood samples of 160 males and females from each ethnic group with no age restrictions ...  Read More

Biological sciences
The golden doses of anti CD3/CD28 microbeads plus IL-15 promote expansion of specific T cells in human cytomegalovirus adaptive immunotherapy

Arsalan Jalili; Nasser Aghdami; Abbas Hajifathali; Maryam Sayahinouri

Volume 7, Issue 1 , July 2023, , Pages 22-45

https://doi.org/10.22034/HBB.2023.09

Abstract
  In this study, the use of anti-CD3/CD28 magnetic microbeads and IL-15 in the culture of T lymphocytes in vitro had a significant role in the proliferation and cytotoxicity of these cells. Peripheral blood mononuclear cells of 6 (Cytomegalovirus) HCMV positive volunteers were harvested, then the pure ...  Read More

Biological sciences
Polyherbal formulation: An alternative therapeutic approach to treat diabetic foot ulcer

Debasmita Dubey; Shakti Rath; Rajesh Kumar Meher; Siba Prasad Dalai; Jayant Kumar Dash; Santosh Kumar Swain

Volume 6, Issue 4 , March 2023, , Pages 1-13

https://doi.org/10.22034/HBB.2023.01

Abstract
  Diabetic Foot Ulcers (DFUs) are a common complication of poorly controlled diabetes and often become infected, termed diabetic foot infection. Despite using several multidisciplinary techniques, no significant cure is still achievable. So, it needs an alternative therapeutic approach to treat diabetic ...  Read More

Biological sciences
Induction and evaluation of influenza cell-mediated immune responses by HA2 subunit and bio adjuvant as a gene vaccine candidate

Sina Soleimani; Mohammadreza Ghorani

Volume 6, Issue 4 , March 2023, , Pages 56-66

https://doi.org/10.22034/HBB.2023.05

Abstract
  The stalk domain of influenza as Hemagglutinin 2 (HA2) is a conserved unit of HA and is a candidate for a vaccine construct. We designed HA2/Mx (Myxovirus Resistance) as bio adjuvant construct for vaccine candidates. After immunization of Razi Balb/c mice in DNA/prime/boost strategy, for determination ...  Read More

Biological sciences
Whole exome sequencing reveals the first c.7456C>T p.Arg2486X mutation in ATM gene in Iranian population

Saeed Dorgaleleh; Karim Naghipoor; Teymoor Khosravi; Amin Tadayoni Nia; Elham Sheikhi Ghayur; Hamayon abdul Aziz; Morteza Oladnabi

Volume 6, Issue 3 , December 2022, , Pages 57-72

https://doi.org/10.22034/HBB.2022.23

Abstract
  Mutations in Ataxia-Telangiectasia Mutated (ATM) gene are prominently responsible for the condition. ATM gene encodes a serine/threonine protein kinase, a crucial component in DNA repair systems. Whole exome sequencing was performed on a nine year old male subject with clinical features of A-T. Alpha ...  Read More

Biological sciences
Ectodermal Dysplasia patients susceptible to COVID-19

Saeed Dorgaleleh; Karim naghipoor; Morteza Oladnabi

Volume 6, Issue 2 , September 2022, , Pages 78-80

https://doi.org/10.22034/HBB.2022.18

Abstract
  Ectodermal dysplasia (ED) is a rare heterogeneous disorder. Defects in the development of the ectoderm cause symptoms in tissues derived from the ectoderm layer such as skin, nails and hair. One of the genes involved in ED is Nuclear Factor Kappa-B (NF-κB) that mutation in this gene causes immunodeficiency. ...  Read More

Biological sciences
Biotransformation of atropine, 4-hydroxy coumarin, 8-hydroxy quinoline, indole and penicillin G by the natural microflora

Seyedeh Mahsa Hosseini; Raheem Haddad; Soroush Sardari

Volume 6, Issue 1 , June 2022, , Pages 13-25

https://doi.org/10.22034/HBB.2022.08

Abstract
  It has been found that natural microorganism have a siginificant role in the production and development of new medicines. The present study aimed to evaluat biotransformation of atropine, 4-hydroxy coumarin, 8-hydroxy quinoline, indole and industrially compound (penicillin G), arrive in the human body, ...  Read More

Biological sciences
The effect of Ajuga reptans extract on in vitro maturation of rat oocytes

Tina mandegarfard; Zahra Arabi; Mahsa Nejati; Nika Mashhadizadeh; Mina Nikandish; Mehdi Ahmadifar

Volume 6, Issue 1 , June 2022, , Pages 33-52

https://doi.org/10.22034/HBB.2022.10

Abstract
  Resumption of nuclear maturation and cytoplasmic changes occur during in vitro maturation. In vitro maturation of the egg provides a stage called Metaphase II (MII) that is required for in vitro fertilization. This study aimed to investigate the effect of replacing Follicle-Stimulating Hormone (FSH) ...  Read More

Biological sciences
Inflammation and Colorectal Cancer: The Bioinformatics Analysis of Multifunctional Role of Microbiota and Diet in Colorectal Cancer Development

Mohammad Ali Pahlevan Neshan; Payam Shahnazi Gerdehsang; Ahmad Bereimipour; Nazila Bostanshirin; Haniyeh Sadat Hosseininia; Kaykhosro Moridi; Amin Ebrahimi Sadrabadi; Arsalan Jalili

Volume 5, Issue 4 , March 2022, , Pages 1-25

https://doi.org/10.22034/HBB.2022.01

Abstract
  Chronic inflammation in colorectal cancer can result in the progression of tumorigenesis. Inflammation in the colorectal part of the body can be affected by microbiota, as well as an individual diet. These two factors play a critical role in cancer types related to the colon and rectum. This inflammation ...  Read More

Biological sciences
The role of different genes in causing infection of ectodermal dysplasia patients: A systematic review

Saeed Dorgaleleh; Zahra Toutounchi; Mahsa Soltani; Karim naghipoor; Morteza Oladnabi

Volume 5, Issue 4 , March 2022, , Pages 86-104

https://doi.org/10.22034/HBB.2022.05

Abstract
  Ectodermal dysplasia (ED) is a genetic disorder which is characterized by deficient tissues from ectoderm and mesoderm. In this systematic review, two researchers searched keywords such as Ectodermal Dysplasia and Hypohidrotic Ectodermal Dysplasia (HED) in Medline (PubMed), Scopus, and Web of Science ...  Read More

Biological sciences
Adjustment of expression of TUBB8, SOX9 and BCL2 genes in apoptosis and polycystic ovarian cancer

Mehdi Ahmadifar; Mina Nikandish; Nika Mashhadizadeh; Mohammad Hossein Azhir; sadaf reihani; ghazal hakiminezhad

Volume 5, Issue 4 , March 2022, , Pages 105-154

https://doi.org/10.22034/HBB.2022.06

Abstract
  Polycystic Ovary Syndrome (PCOS) is the most common heterogeneous Endocrine (EC) disorder in women of childbearing age, which has an increasing trend in the number of patients recently. Although women with PCOS have been shown to triple the risk of EC compared to women without polycystic ovary syndrome, ...  Read More