Document Type : Original Research Article

Authors

• Tina mandegarfard ¹
• Zahra Arabi ²
• Mahsa Nejati ³
• Nika Mashhadizadeh ³
• Mina Nikandish ³
• Mehdi Ahmadifar ⁴

¹ 1. Department of biology, college of science, University of science and culture, ACECR, Tehran branch, Iran.

² 2. Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.

³ 1. Department of biology, college of science, University of science and culture, ACECR, Tehran branch, Iran

⁴ 3. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

Abstract

Resumption of nuclear maturation and cytoplasmic changes occur during in vitro maturation. In vitro maturation of the egg provides a stage called Metaphase II (MII) that is required for in vitro fertilization. This study aimed to investigate the effect of replacing Follicle-Stimulating Hormone (FSH) with plant extract that 5 groups were used. The null group, which consisted only of oocytes, the control group, which used Fetal Bovine Serum (FBS) and FSH, the 3 experimental groups, in which different doses of Ajuga L. extract were substituted for FSH. Ajuga extract with appropriate dose increased the prevalence of Germinal Vesicle Degradation (GVBD), growth rate and maturity of immature mouse oocytes to MII stage.

Keywords

• Ajuga L
• maturation
• germinal vesicle degradation

Main Subjects

• Biological sciences