Document Type : Original Research Article

Authors

• Saghar Bakhtiari ¹
• Fatemeh Noorbakhsh ¹
• Sadaf Mohseni Fard ¹
• Raheleh Safaei javan ²

¹ Department of Microbiology, Biological Sciences College, Varamin-Pishva Branch, Islamic Azad University, Varamin-Pishva, Iran

² Department of Biochemistry and Biophysics, Varamin-Pishva Branch, Islamic Azad University, Varamin-Pishva, Iran

Abstract

The aim of this research was to investigate the effect of Zataria multiflora and silver nanoparticles on the biofilm formation ability of A. baumannii and the expression of genes involved in this process. Thirty isolates of A. baumannii bacteria were collected and studied from the Milad Hospital of Tehran. The effects of Zataria multiflora essential oil and silver nanoparticles on the biofilm formation process were analyzed using the microtiter plate method. Also, the gene expressions of pil and aba after exposure to nanoparticles and essential oil were measured. Treatment the strains with Zataria multiflora essential oil at the MIC concentration, along with silver nanoparticles, reduced biofilm formation by the strains assessed and the expression of the genes noted. It seems that Z.multiflora essential oil and silver nanoparticles, can be used as candidate drugs in treatment or disinfection.

Keywords

• Acinetobacter baumannii
• biofilm
• essential oil
• silver nanoparticles

Main Subjects

• Biological sciences