Document Type : Original Research Article


1 Razi Vaccine & Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), P.O. Box 31975-148, Karaj, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Postal Code: 5166616471, Tabriz, Iran



The stalk domain of influenza as Hemagglutinin 2 (HA2) is a conserved unit of HA and is a candidate for a vaccine construct. We designed HA2/Mx (Myxovirus Resistance) as bio adjuvant construct for vaccine candidates. After immunization of Razi Balb/c mice in DNA/prime/boost strategy, for determination of the cellular immunity by an in vivo assay, the lymphocyte proliferation was evaluated. The result indicated the most stimulation index was by HA2 and Mx in three boosters, and effective stimulation of cellular immunity was obtained. No apparent side effects and histopathology were observed. Due to this result in cellular immunity and appropriate increase in humoral immunity that was obtained in our previous study, as well as the absence of antigen and adjuvant side effects, this construct can be introduced as a suitable candidate for developing an influenza vaccine.


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