Document Type : Original Research Article


1 Department of Applied Cell Sciences, Faculty of Basic Sciences and Advanced Medical Technologies, Royan Institute, ACECR, Tehran, Iran

2 Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran

3 Hematopoeitic Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

4 Department of Immunology, Afzalipour Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran



In this study, the use of anti-CD3/CD28 magnetic microbeads and IL-15 in the culture of T lymphocytes in vitro had a significant role in the proliferation and cytotoxicity of these cells. Peripheral blood mononuclear cells of 6 (Cytomegalovirus) HCMV positive volunteers were harvested, then the pure population of lymphocytes were isolated. HCMV-Specific T Cells were expanded in 4 groups consisting of IL-2, IL-15 and anti-CD3/CD28 microbeads. A significant effect of IL-15 along with anti-CD3/CD28 magnetic microbeads on the proliferation rate of HCMV-specific T lymphocytes was evaluated.


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