Document Type : Original Research Article
1 Department of Applied Cell Sciences, Faculty of Basic Sciences and Advanced Medical Technologies, Royan Institute, ACECR, Tehran, Iran
2 Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran
3 Hematopoeitic Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4 Department of Immunology, Afzalipour Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Background: There are various mechanisms are being developed to expand Virus specific T lymphocytes. In this study, the use of anti-CD3/CD28 magnetic microbeads and IL-15 in the culture of T lymphocytes in vitro has a significant role in the proliferation and cytotoxicity of these cells.
Materials and Methods: Peripheral blood mononuclear cells of 6 HCMV positive volunteers were harvested, then the pure population of lymphocytes were isolated. HCMV-Specific T Cells were expanded in 4 groups consisting of IL-2, IL-15 and anti-CD3/CD28 microbeads. Cells were assessed on the 14th day.
Results: A significant effect of IL-15 along with anti-CD3/CD28 magnetic microbeads on the proliferation rate of HCMV-specific T lymphocytes, and also increase the cytotoxicity and the secretion of gamma interferon.
Conclusion: The use of IL-15 along with anti-CD3/CD28 beads shows effective results for virus specific T cell proliferation.