Abstract
Platelets are thought to aid in hemostasis, thrombosis, inflammation, wound healing, and immunity. R4 is a novel thiazole derivative that may decrease angina and ischemia. An aggregometer evaluated R4 impact on PRP. Spectrophotometric method measured anti-lipoxygenase activity of R4. In its absence, ...
Read More
Platelets are thought to aid in hemostasis, thrombosis, inflammation, wound healing, and immunity. R4 is a novel thiazole derivative that may decrease angina and ischemia. An aggregometer evaluated R4 impact on PRP. Spectrophotometric method measured anti-lipoxygenase activity of R4. In its absence, the platelet aggregation in PRP showed 88 % and 75 % with collagen and ADP, respectively, while R4 inhibited 100 % collagen-induced platelet aggregation at 1.1875 μM and ADP-induced platelet aggregation up to 100 % at 0.9375 μM. Collagen and ADP induced R4 antiplatelet IC50 values were 0.55 ± 0.12 μM, and 0.26 ± 0.20 μM, respectively. R4 inhibited lipoxygenase significantly with an IC50 of 26.65 ± 0.16 μM. The novel compound, R4, may also assist platelet-associated thromboembolic disorders.
Abstract
In this study, we investigated the anti-platelet activity of bergenin on platelet aggregation induced by collagen and ADP on blood sample of healthy volunteers (18–40 years). Blood sample was centrifuge and Platelet Rich Plasma (PRP) was carefully collected in tube. Lipoxygenase inhibition activity ...
Read More
In this study, we investigated the anti-platelet activity of bergenin on platelet aggregation induced by collagen and ADP on blood sample of healthy volunteers (18–40 years). Blood sample was centrifuge and Platelet Rich Plasma (PRP) was carefully collected in tube. Lipoxygenase inhibition activity of bergenin was conducted by spectrophotometric method. In the absence of bergenin about 84 % platelet aggregations showed in PRP, while in its presences 100 % inhibition of platelet aggregation at 6.25 mM induced by collagen. While with the same concentration of bergenin, inhibited platelet aggregation was 91.88 % ± 5.14 induce by ADP. The IC50 values of antiplatelet effect of bergenin induced by collagen and ADP were 3.07mM ± 0.22 and 3.32mM ± 0.10, respectively. The study indicates that bergenin has potent antiplatelet activity to collagen and ADP induced aggregation.