Biological sciences
Seyed Mohammad Haji Mirghasemi; Hamid Reza Pordeli; Leila Fozouni; Mina Komshikamar; fatemeh komshikamar; Mansoor Rubin
Abstract
In this study, eight bacterial isolates were obtained from the 35 soil samples of Golestan province in Iran. Samples were collected from different soils and cultured in an M9 medium. Enzyme-producing colonies were distinguished by the color change from yellow to pink. 16s rRNA sequencing identified L-asparaginase ...
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In this study, eight bacterial isolates were obtained from the 35 soil samples of Golestan province in Iran. Samples were collected from different soils and cultured in an M9 medium. Enzyme-producing colonies were distinguished by the color change from yellow to pink. 16s rRNA sequencing identified L-asparaginase (L-ASNase)-producing bacterial species in soil samples. The presence of L-ASNase in Bacillus subtilis was shown by M9 culture and confirmed by spectrophotometry. Bacillus subtilis was identified by 16S rRNA gene sequencing. L-ASNase isolated from Bacillus subtilis showed effective antioxidant activity and also anti-bacterial activity against Staphylococcus aureus and Escherichia coli. The present study demonstrated the presence of L-ASNase-producing Bacillus subtilis strain in soil samples with antioxidant and antibacterial activity.
Pharmaceutical sciences
Yoga Windhu Wardhana; Bayu Indradi; Ade Zuhrotun; Rini Hendriani; Winda Agnestia Maranna Saragih; Savira Ekawardhani; Hesti Lina Wiraswasti; Enny Rohmawaty; Sumartini Dewi
Abstract
In this study, thermal stability and pH for the antioxidant activity of the ethanol extract of ciplukan (Physalis angulata Linn) herbs had been studied. Among the antioxidant activity methods, the use of DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent is simple and easy to use. The thermal effect was observed ...
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In this study, thermal stability and pH for the antioxidant activity of the ethanol extract of ciplukan (Physalis angulata Linn) herbs had been studied. Among the antioxidant activity methods, the use of DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent is simple and easy to use. The thermal effect was observed by warming the sample at 50, 70, and 100 °C in intervals of 30, 45, and 60 min. The results showed that the half-maximal Inhibitory Concentration (IC50) of the extract was 185.53+1.35, which was classified as medium. Based on these results, a significant decrease was observed at 100 °C and pH=8. Thus, the pKa of the extract is recommended for maintaining stability at a pKa value of 3.83, which is proven to maintain a spot in the pH range of 4.
