Document Type : Original Research Article

Authors

• Yoga Windhu Wardhana ¹
• Bayu Indradi ²
• Ade Zuhrotun ²
• Rini Hendriani ³
• Winda Agnestia Maranna Saragih ⁴
• Savira Ekawardhani ⁵
• Hesti Lina Wiraswasti ⁵
• Enny Rohmawaty ⁶
• Sumartini Dewi ⁷

¹ Dosage Form Development Research Center, Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Padjadjaran

² Center for Herbal Studies, Department of Biological Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, Indonesia

³ Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, Indonesia

⁴ Department of Internal Medicine, Faculty of Medicine, Padjadjaran University, Sumedang, Indonesia

⁵ Department of Biomedical Science, Faculty of Medicine, Universitas Padjadjaran, Sumedang, Indonesia

⁶ Division of Pharmacology and Therapy, Department of Biomedical Sciences, Faculty of Medicine, Uni-versitas Padjadjaran, Sumedang, Indonesia

⁷ Immunology Study Center, Division Rheumatology, Department of Internal Medicine, Faculty of Medicine, Padjadjaran University, Sumedang, Indonesia

Abstract

In this study, thermal stability and pH for the antioxidant activity of the ethanol extract of ciplukan (Physalis angulata Linn) herbs had been studied. Among the antioxidant activity methods, the use of DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent is simple and easy to use. The thermal effect was observed by warming the sample at 50, 70, and 100 °C in intervals of 30, 45, and 60 min. The results showed that the half-maximal Inhibitory Concentration (IC₅₀) of the extract was 185.53+1.35, which was classified as medium. Based on these results, a significant decrease was observed at 100 °C and pH=8. Thus, the pKa of the extract is recommended for maintaining stability at a pKa value of 3.83, which is proven to maintain a spot in the pH range of 4.

Keywords

• Ciplukan (Physalis angulata Linn.)
• antioxidant activity
• temperature

Main Subjects

• Pharmaceutical sciences