Masoud Salehipour; Masoumeh Karimi
Abstract
Genetic disorders play an important role in male infertility, especially in Non-Obstructive Azoospermia (NOA). Many studies have been performed on the expression profiles of gonadal genes in order to accurately identify the genes involved in impaired spermatogenesis. In the current study, testicular ...
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Genetic disorders play an important role in male infertility, especially in Non-Obstructive Azoospermia (NOA). Many studies have been performed on the expression profiles of gonadal genes in order to accurately identify the genes involved in impaired spermatogenesis. In the current study, testicular biopsy, hormonal assay, and Real-Time PCR were done to check hormonal and gene expression levels of the CAPN11 and UBQLN3 genes in the testicular biopsy samples of 30 NOA and 30 Obstructive Azoospermia (OA) patients. The Real-time PCR showed that the expression of these genes decreased in azoospermia patients. The hormonal evaluation showed that FSH and LH significantly increased in NOA men and there was no difference in testosterone level between the two groups. Due to the obtained results of this study, CAPN11 and UBQLN3 can introduce as two biomarkers of NOA.
Abstract
Male infertility related to diabetes is under investigation to find a proper therapeutic strategy with minimal side effects. Using Nigella sativa as a supplementary agent could be helpful. This study was designed to know Nigella sativa effect on hormone profiles and histology of diabetes type II model. ...
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Male infertility related to diabetes is under investigation to find a proper therapeutic strategy with minimal side effects. Using Nigella sativa as a supplementary agent could be helpful. This study was designed to know Nigella sativa effect on hormone profiles and histology of diabetes type II model. 40 male rats (250-300 g) were divided into four groups which diabetic rats received 200 and 400 mg/kg extract of Nigella sativa for 90 days. The male hormones profiles, weight and the histopathological configuration were analyzed at end of study. Nigella sativa improved the hormones profile in experimental groups. Also, the number of germinal epithelium increased in experimental groups following Nigella sativa administration. Although, therapeutic effects of Nigella sativa are promising; several preclinical and clinical trials are necessary to optimize its proper dose and time in remedy of male infertility.
