Our study was designed to evaluate any changes in the liver, kidney, and brain tissues of female wistar rats to a fructose-enriched diet. Rats were divided into three groups (n=5/group). Monitoring of daily water-food consumption and clinical changes continued for 90 days. Afterward, the alteration of biochemical, necropsy, and histopathological parameters was assessed on day 91. Increased brain weights, presence of eosinophilic neurons, gliosis, and cerebral congestion were recorded. Also, the decrease in total and non-conjugated serum bilirubin levels, increase in serum glucose level, liver vacuolar degeneration, and single-cell necrosis were shown that obtained results indicated signs of toxicity in the neural tissue of rats.