The aim of this project was to investigate the P53 gene mutations in exon 8 in the patients with astrocytoma type brain tumor. This was an in vitro study, in which the extraction of DNA from 30 samples of brain tissue was performed by phenol-chloroform protocols. After polymerase chain reaction (PCR) amplification of exon 8 of p53 genes, screening by single strand conformation polymorphism (SSCP) was performed to detect the shift. In this study, three malignant novel missense mutations in one sample with grade 4 of astrocytoma were detected. Also one synonymous mutation in grade 4 of astrocytoma was detected. The findings were indicated that mutations in exon 8 of the P53 gene mostly were in malignant astrocytoma brain tumor type, which is important in diagnosis and gene therapy of brain tumors.