Document Type : Original Research Article



Brucella spp., causes zoonotic infections. Our objective was screening and risk factors of Brucella species among human sera samples in Sistan va Baluchistan province of Iran. Fifty three sera samples were positive using indirect Enzyme Linked Immunosorbent Assay (ELISA), but the Wright and 2-ME agglutination tests yielded lower positive cases. Thirty one (58.5 %) samples contained Brucella spp using PCR among which B. abortus and B. melitensis were detected in 25 (80.6 %) and 6 (19.4 %) of specimens, respectively. Risk factors associated with B. abortus and B. melitensis infection included age ranging 25-45 years (23/31), male sex (26/31) and consumption of dairy products (22/31). Consumption of unpasteurized cow milk and livestock exposure were the significant risk factors.


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