The emergence of nosocomial infection due to Multi-Drug Resistant (MDR) bacteria is a challenge to infection control. This study presented two Providencia species from two hospitalized patients admitted to Imam Hussein hospital in Iran. Biochemical and molecular tests were performed for the identification of the strains. The resistance pattern of these isolates was determined using an antimicrobial susceptibility test. Both P. stuartii isolates were resistant to all antibiotics. Among carbapenemase resistance genes, blaNDM, blaGES and blaKPC were detected in both of them. One isolate was able to transfer the resistant gene in the conjugation test. The presence of MDR strains among nosocomial infection agents such as P. stuartii isolates could affect the health care system efficiency.