Document Type : Original Research Article
Authors
- Maryam Naderi Mezajin 1
- Mahdi Fatemizadeh 2
- Zahra Rostami 3
- Pejvak Khaki 4
- Masoomeh Shirzad 5
- Fatemeh Noorbakhsh 6
1 Department of Microbiology, Varamin-Pishva Branch, Islamic Azad Univercity, Varamin-Pishva, Iran
2 Department of Neuroscience and Addiction Studies, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
3 Department of Microbiology, Islamic Azad University Science and Research Branch , Islamic Azad Univercity, Tehran, Iran
4 Department of Microbiology, Razi Vaccine and serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
5 Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
6 Department of Microbiology, Biological Science College, Varamin-Pishva Branch, Islamic Azad Univercity, Varamin-Pishva, Iran
Abstract
The aim of this study was to investigate the antibiotic resistance and identification of strains producing broad-spectrum beta-lactamases salmonella strains. The 70 different strains of salmonella were studied that gentamicin, ciprofloxacin, ofloxacin, imipenem, and enrofloxacin were susceptible. The highest resistance to cephalexin (96 %), cefazolin (96 %), cephalothin (65 %), and 69 isolates (98 %), were resistant to more than one antibiotic. The results of the Polymerase Chain Reaction (PCR) were evaluated on ESBL producing isolates using specific primers; in order to investigate the presence of CTX-Mbla, SHVbla, TEMbla and OXAbla, so the presence of germ cell resistance genes in total indicated that 32 isolates (56 %), at least one of the above genes.
Keywords