The aim of this study was to investigate the frequency of hepatitis C virus genotypes with chronic hepatitis C referred to Nikopour clinic of Yazd during 2010-2020. In this cross-sectional study RT-PCR was used to determine the genotype of hepatitis C virus. The patient information was recorded in a questionnaire and finally the data were analyzed by SPSS software. During this study, 104 patients with hepatitis C were evaluated with a mean age of 42.9 years. The most common hepatitis C virus genotypes were 3a, 1a and 3, respectively. The HCV genotype was significantly associated with risk factor and history of blood transfusion in patients. Considering that the predominant genotype is 3a and about 35.6 %, seems that genotype determination would be a candidate for treatment.