Genetic variations in Interleukin-10 (IL-10), an anti-inflammatory cytokine, has the potential to influence the development of Ischemic Stroke (IS). The current study aimed to examine IL-10 gene polymorphisms (rs1800896, rs1800871 and rs1800872) in association with IS. After genotyping, associational analyses showed a significant correlation of rs1800896 with the elevated risk of disease. Whereas for rs1800871, a decreased risk was observed and it might play a protective role. However, rs1800872 did not reveal an association with the predisposition to IS. Furthermore, all the haplotypes were significantly associated with the disease risk except GCA. The acquired Dʹ value from linkage disequilibrium showed the tendency of co-inheritance. Thus, the obtained results suggested that rs1800896 and rs1800871 have a significant relationship with IS.