Snake bite was known as one of the most important public health concerns world wild. Respiratory and cardiac problems and destruction of endothelium system occurs as a result of snake bite. Some proteins and peptides in snake venom have potential for drug discovery studies. According to the important role of cell adhesion and angiogenesis in cancer development, identification of new therapeutics that targets this process seems indispensable. Evaluation of cytotoxicity, anti-adhesive and anti-angiogenic effects of Caspian Cobra snake (Naja oxiana) venom on human endothelial cell was the main aim of current study. Methods: Crude Cobra snake venom was fractionated by fast protein liquid chromatography (FPLC) using S-200 column. Purity of crude venom and each fraction was monitored by SDS-PAGE. Cytotoxicity of Caspian Cobra snake (Naja oxiana) crude venom and fractions on human endothelial cells was evaluated by MTT assay Adhesion and tube formation assay was performed to evaluate anti-adhesive and anti-angiogenic activities of snake venom. Results of FPLC revealed eight individual fractions. Cobra crude venom and fractions showed dose-dependent cytotoxic effect on human endothelial cell. Fraction 6 (IC50=5.7 μg/ml) and fraction 7 (IC50=5 μg/ml) showed higher cytotoxic effects on human endothelial cells. Therefore, further assays carried out with fraction 6 (F6) and 7 (F7) and results showed that both of fractions inhibited in vitro adhesion and tube formation of human endothelial cellsResults evaluated cytotoxicity, anti-adhesive and anti-angiogenic effects of Caspian Cobra snake (Naja oxiana) venom on human endothelial cell and represents promising tool for drug discovery and development.