Document Type : Original Research Article

Abstract

Breast cancer is 26% of diagnosed cancer in 2008 and it is second cause of cancer related deaths in women. This type of cancer is a complex genetic disease, because microRNAs (miRNAs) are expressional regulators of genes, genetic variations such as single nucleotide polymorphisms in their genes. Therefore, this study was conducted to evaluate the association between single nucleotide polymorphisms (SNPs) in miRNA 148/152 genes and breast cancer risk in Isfahan population. This case control study was performed on 200 samples containing 100 controls and 100 cases. Polymorphism genotyping was performed using polymerase chain reaction restriction fragment length polymorphism (PCR–RFLP) and tetra primer amplification refractory mutation system (T-ARMS-PCR).
Our observations indicated no significant association between rs185641358 and rs12940701 in miRNAs and breast cancer risk because their p-values are 0.08 and 0.19, respectively. No association between miRNA148/152 genes polymorphisms and risk of breast cancer was found.

Keywords

• Breast cancer
• miRNA148a
• miRNA152
• SNP