The experimental conditions were investigated to fabricate Au nanorod (NRs) by pulsed laser ablation in water. This study revealed that it is possible to produce alternatively nanoparticles or nanorod at certain wavelengths depending on the laser. The values of the laser fluence for both wavelengths under the experimental conditions were chosen from several J/cm2 to tens of J/cm2. The optical extinction spectra of the colloids in the UV-Vis and IR regions were obtained to evaluate the structure of the dispersed Au phase. Scanning electron microscopy (SEM) was applied to visualize the size and morphology of the colloidal particles. Fourier transform infrared spectroscopy (FTIR) spectra of the samples were recorded.