The aim of the present study was to reveal a part of genetic profile of multiple sclerosis (MS) in Khuzestan province. The contribution of HLA-DR2 haplotype (HLA-DRB1*1501-DQA1*0102-DQB1*0602) to MS risk is confirmed. This study included 187 MS patients and 192 healthy individuals. HLA alleles were genotyped, using the polymerase chain reaction amplification with sequence specific primers (PCR-SSP) method. The statistical analyses were performed by SPSS software. The results of this study demonstrated that distribution of HLA-DQA1*0102--DQB1*0602+ haplotype was statistically different between patients and controls. HLA-DRB1*1501+-DQA1*0102- was more frequent among women although DQA1*0102+-DQB1*0602+ showed negative association of female patients compare with female controls. Significant correlation was observed among DRB1*1501+-DQA1*0102- with Fars group. HLA-DRB1*1501+-DQA1*0102+ haplotype was positively associated with EDSS steps 5 to 10. These results suggest that HLA-DRB1*1501+-DQA1*0102- haplotype may involve in MS susceptibility among women.