The glutathione S-transferase (GST) is a polymorphic supergene family of detoxification enzymes that are involved in the metabolism of potential carcinogens. To evaluate the association between polymorphisms in the GSTs (GSTT1, GSTM1 and GSTP1) and basal cell carcinoma (BCC), 50 BCC patients and 50 healthy controls were studied. Based on our findings, the frequencies of GSTM1 null genotype in patients and controls were 34 % and 6 %, respectively. Furthermore, the overall frequency of GSTT1 null genotype was higher than in the BCC patients compared to the control samples. The strongest association was observed between GSTP1 polymorphism and BCC in the population. Genotype mixture of GSTM1 and GSTT1 showed a higher risk of developing BCC in subjects with one null genotype. This study suggested a reasonable association between GST polymorphisms and BCC susceptibility among Iranian population.