Increased application of electrical devices and consequent expansion of electrical distribution network, expose us to extremely low frequency electromagnetic fields (ELF-EMF) more than ever. Mechanisms of action of ELF-EMFs on cells and biomolecules are not well understood. Presence of specific sequence for responsiveness of DNA to ELF-EMF exposure are demonstrated by several in vivo studies. In the present study, possibility of conformational transition and denaturation of double-stranded oligonucleotides with different sequences which exposed to ELF-EMF were examined by circular dichroism (CD) spectroscopy. The data obtained from this study did not provide any evidence in support of significant change in double-stranded oligonucleotides conformation.