Drug discovery from the venom of venomous animals is one of the marvelous and cost effective ways.Snake venom is one of the important sources of drug discovery. There are various active biomolecules in the snake venom. N.oxiana venom fractionated and cytotoxic effect of each fraction was examined by MTT assay. Antitumor activity of N. oxiana venom fractions were evaluated using TC-1 tumor bearing mouse. Results indicate for anti-tumor activity of some fractions of N. oxiana venom on TC-1 tumor proliferation and growth. Taken together, observed results indicate for potential of N. oxiana as drug discovery source for cancer therapy.