Health Biotechnology and BiopharmaHealth Biotechnology and Biopharma (HBB)2538-44144320201201The combination effects of theophylline and corticosteroids in COVID-191513305110.22034/HBB.2020.13ENJournal Article20210705Late in 2019, the novel coronavirus disease (COVID-19) became pandemic. The disease has associated with severe inflammatory symptoms of the respiratory epithelial cells and the dysfunction of several organs of the body. Studies have shown that theophylline plays an important role in acute inflammation and has a synergistic effect on low therapeutic concentrations with corticosteroid drugs and amplifies anti-inflammatory effect of corticosteroids by activating histone deacetylase-2 (HDAC2), which decreases corticosteroid resistance by increasing the affinity of corticosteroid receptors to corticosteroid drugs. Therefore, theophylline could be considered as an adjunctive anti-inflammatory drug in combination with corticosteroids in the treatment of patients with COVID-19.https://www.healthbiotechpharm.org/article_133051_572fef1e695dd956577f01814c87a299.pdfHealth Biotechnology and BiopharmaHealth Biotechnology and Biopharma (HBB)2538-44144320201201Investigation of chemical components and antibacterial activity of yarrow (Achillea wilhelmsii) extract61813305210.22034/HBB.2020.14ENJournal Article20210705Herbal medicine is one of the oldest human approaches to treating diseases and yarrow (Achillea wilhelmsii) one of the most famous medicinal plants. Yarrow essential oil has anti-inflammatory, anti-spasm, anti-scalp, hair growth stimulation and topical skin healing due to chamazulene, cineole and borneol. Staphylococcus aureus (S.aureus) and Escherichia coli (E.coli) are the most important bacteria causing nosocomial infections with multiple antibiotic resistances. Also, S.aureus strain is more sensitive than E.coli to extract of yarrow. Alpha-pinene, phenolic compounds and flavonoids are major constituents of yarrow. This review shows how methanol extract of yarrow prevented the growth of Gram-positive bacteria such as S.aureus and E.coli. So these extracts can be substituted for chemical drugs.https://www.healthbiotechpharm.org/article_133052_254c9747f8e70a258db2767ff3d50baa.pdfHealth Biotechnology and BiopharmaHealth Biotechnology and Biopharma (HBB)2538-44144320201201Quantitative evaluation of DYT1 promoter methylation in dystonia patients192713305310.22034/HBB.2020.15ENJournal Article20210705This study aimed at assessing the methylation state of the DYT1 gene in the pathogenesis of dystonia disease and evaluated the clinical feature. Thirty patients with no mutation in DYT1 gene and 30 normal individuals were investigated. The methylation of promoter in DYT1 gene was investigated using Real-time PCR technique. There was no significant difference in methylation status of DYT1 gene promoter in the dystonia patients compared with normal individuals, showed that the methylation of promoter in DYT1 gene had no important role in dystonia. Also, the methylation of promoter in DYT1 gene was observed with higher frequency in the generalized type, suggesting that the methylation of this gene may play a role in pathogenesis of this type of dystonia.https://www.healthbiotechpharm.org/article_133053_60cad6da49b5fc404a92c69c9711c4f8.pdfHealth Biotechnology and BiopharmaHealth Biotechnology and Biopharma (HBB)2538-44144320201201Effect of spices and aromatic compounds on inhibit COVID_19 Mpro by docking analysis284613305410.22034/HBB.2020.16ENJournal Article20210705Coronavirus 2019 is a disaster in the 21st century and the lack of specific medications for prevention or treatment is a major need. In this regard, we conducted a conventional study to identify the main targets of coronavirus among spices compound. For this bioinformatics study, the ingredients structure was obtained from the PubChem database and the protease enzyme structure COVID_19 from the PDB database then molecular docking was performed by Molegro Virtual Docker (MVD) software. Based on the results, it could be concluded that the spices compounds interfere with the important amino acids in the enzymatic cavity to inhibit the protease enzyme virus. Regarding the binding energy among these compounds, the metabolites of curcuma longa, capsicum, zingiber has the strongest binding and inhibitory effect on the protease enzyme COVID_19. Therefore, these spices could be prescribed as a complementary treatment for patients with coronavirus infection in their food, but this study requires clinical investigation.https://www.healthbiotechpharm.org/article_133054_4c14ad43bdef03e951090ce5ccb22280.pdfHealth Biotechnology and BiopharmaHealth Biotechnology and Biopharma (HBB)2538-44144320201201Prevalence and risk factors associated with Brucella abortus and Brucella melitensis human infections in Sistan va Baluchistan province, southwest of Iran475513305510.22034/HBB.2020.17ENJournal Article20210705Brucella spp., causes zoonotic infections. Our objective was screening and risk factors of Brucella species among human sera samples in Sistan va Baluchistan province of Iran. Fifty three sera samples were positive using indirect Enzyme Linked Immunosorbent Assay (ELISA), but the Wright and 2-ME agglutination tests yielded lower positive cases. Thirty one (58.5 %) samples contained Brucella spp using PCR among which B. abortus and B. melitensis were detected in 25 (80.6 %) and 6 (19.4 %) of specimens, respectively. Risk factors associated with B. abortus and B. melitensis infection included age ranging 25-45 years (23/31), male sex (26/31) and consumption of dairy products (22/31). Consumption of unpasteurized cow milk and livestock exposure were the significant risk factors.https://www.healthbiotechpharm.org/article_133055_9ec2e29d169fadd6830fa5cf89673bfe.pdfHealth Biotechnology and BiopharmaHealth Biotechnology and Biopharma (HBB)2538-44144320201201Tracking of intraperitoneally and direct intrahepatic administered mesenchymal stem cells expressing miR-146a-5p in mice hepatic tissue567613305710.22034/HBB.2020.18ENJournal Article20210705Given the functional drawbacks in the efficacy and safety of conventional miRNA delivery approaches, we aimed to assess the fate of mesenchymal stem cells (MSCs) expressing miR-146a-5p into the mice hepatic tissue. MSCs were xeno-transplanted through direct intrahepatic and intraperitoneal routes into immunocompetent and cyclosporine A treated BALB/c mice. DNA extracts from murine organs 24 h and 28 days after xenotransplantation were analyzed by PCR method for detection of human-specific Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) sequence. Xeno-transplanted naive MSCs could not be detected after 24 h and 28 days post-infusion at the various organs of mice. Considering the miR-146a-5p expression in MSCs together with negative results of cell engraftment in hepatic tissue, the present study suggests the cell-free secretome-based miR-146a-5p delivery for modulation of altered miRNA expression in hepatic stellate cells.https://www.healthbiotechpharm.org/article_133057_1ab263e5d27aafd48b3d7958a4ac2e2e.pdf