Samira Beyramy; Soroush Sardari
Abstract
Erythropoietin (EPO) is one of the most important hormones in the human body, due to its effective pharmaceutical performance, recombinant human EPO (rhEPO) is often produced by Chinese Hamster Ovary (CHO) cells as host cells through recombinant DNA technology on an industrial scale. In this study, the ...
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Erythropoietin (EPO) is one of the most important hormones in the human body, due to its effective pharmaceutical performance, recombinant human EPO (rhEPO) is often produced by Chinese Hamster Ovary (CHO) cells as host cells through recombinant DNA technology on an industrial scale. In this study, the reported genome-scale metabolic network of CHO cells was upgraded to integrate EPO production pathways using the INIT algorithm in the RAVEN Toolbox. After quality analysis for the reconstructed model, performance of the model was examined under two different culture conditions provided within the literature. Such analysis were implemented through Flux Balance Analysis (FBA) and Multi-objective Analysis techniques and the results highlighted the effectiveness of these culture conditions. To enhance the efficiency of rhEPO production, analysis of essential genes and reactions, sensitivity of essential amino acid supplementation and flexibility of amino acid uptakes was also performed through a series of standard in silico techniques in constraint-based analysis.
Pharmaceutical sciences
Noor Adnan Najm; Ekhlas Sabah Hassan
Abstract
Sepsis is now more commonly regarded as an uncontrolled inflammatory and immune response throughout the body, triggered by microbial invasion and resulting in high mortality rate from organ damage. This study aimed to demonstrate the protective impact of ipragliflozin on lung damage induced in mice by ...
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Sepsis is now more commonly regarded as an uncontrolled inflammatory and immune response throughout the body, triggered by microbial invasion and resulting in high mortality rate from organ damage. This study aimed to demonstrate the protective impact of ipragliflozin on lung damage induced in mice by Cecal Ligation and Puncture (CLP). The levels of lung tissue inflammatory & oxidative stress markers (IL-1β, IL-6, TNF-α, NF-KB & MDA) were significantly elevated in the sepsis in comparison to the sham. On other hand, mice treated with ipragliflozin had a significant reduction in the level of these mediators. Histological examination revealed significant lung tissue damage in mice undergo sepsis which significantly reduced after ipragliflozin treatment. Ipragliflozin shows promise in reducing pulmonary dysfunction in male mice with sepsis.
Biological sciences
Mehdi Ahmadifar; Mina Nikandish; Nika Mashhadizadeh; Mohammad Hossein Azhir; sadaf reihani; ghazal hakiminezhad
Abstract
Polycystic Ovary Syndrome (PCOS) is the most common heterogeneous Endocrine (EC) disorder in women of childbearing age, which has an increasing trend in the number of patients recently. Although women with PCOS have been shown to triple the risk of EC compared to women without polycystic ovary syndrome, ...
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Polycystic Ovary Syndrome (PCOS) is the most common heterogeneous Endocrine (EC) disorder in women of childbearing age, which has an increasing trend in the number of patients recently. Although women with PCOS have been shown to triple the risk of EC compared to women without polycystic ovary syndrome, there are precise molecular mechanisms that increase the risk of EC in women with ovarian syndrome. Therefore, clinical strategies to prevent EC in PCOS are not well known. Although, elevated estrogen levels and decreased apoptosis have been suggested as potential mechanisms, there is no clarity on how these and other factors interact to increase the risk of EC in polycystic ovary syndrome. In this article, we try to review the functional mechanisms of TUBB8, SOX9 and BCL2 genes to examine their effect on controlling or promoting the risk of EC.
Medicinal sciences
Ani Boghozian; Ali Choopani; Hamid Reza Shiran; Amirhossein Choopani; Nahid Ghaedamini
Abstract
The purpose of this study was to investigate the effect of Artemisia Ouchei on the liver enzymes of post -treatment with paracetamol. Artemisia was collected from Kashan area in Iran. The flower Essential Oil of this plant was extracted using a cloner device and the components of it were separated and ...
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The purpose of this study was to investigate the effect of Artemisia Ouchei on the liver enzymes of post -treatment with paracetamol. Artemisia was collected from Kashan area in Iran. The flower Essential Oil of this plant was extracted using a cloner device and the components of it were separated and identified using gas chromatography mass spectrometry. Four groups of animals were used to measure liver factors: 1- control, 2- paracetamol, 3- paracetamol with essential oil, 4- essential oil only. Paracetamol was injected for 3 days and essential oil for 7 days. ALP, AST, ALT were measured 3 days after the last blood transfusion. Essential oil with paracetamol significantly reduced AST and ALT levels and increased ALP level compared to paracetamol alone. Paracetamol and Artemisia Ouchei both caused liver damage by oxidative stress, but their combined use reduced their toxicity.
Biological sciences
Elaheh Gholami Roudmajan; Ali H. Al-Marzoqi; Miaad K. Alkhudhairy; Maryam Kohansal; Abdolmajid Ghasemian; Seyed Masoud Houshmand
Abstract
A leading risk factor for cervical cancer (CC) initiation and progress includes human papillomavirus (HPV) infection. The telomerase catalytic subunit which regulates senescence and proliferation (carcinogenesis) is encoded by human telomerase reverse transcriptase (hTERT) gene. Our objective was the ...
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A leading risk factor for cervical cancer (CC) initiation and progress includes human papillomavirus (HPV) infection. The telomerase catalytic subunit which regulates senescence and proliferation (carcinogenesis) is encoded by human telomerase reverse transcriptase (hTERT) gene. Our objective was the assessment of high risk HPV genotypes (16, 18 and 31) association with two polymorphisms of hTERT gene (rs2736098 and rs2736100) in CC development. Forty CC specimens were retired and 49 blood samples from healthy individuals were used as control group. The PCR-RFLP was performed for the detection of rs2736098 and rs2736100 polymorphisms. The differences of A, C or G alleles were not significant between case and control. Accordingly, among 10 high risk HPV genotypes, genotype 18 was detected and there was no meaningful relation between neither the hTERT rs2736098 nor the rs2736100 polymorphisms and CC risk.
Biological sciences
Miaad K. Alkhudhairy; Rasha Fadhel Obaid; Haider Qassim Raheem; Ahmad Shaya Kareem; Hamidreza Kianfar; Mojtaba Memariani; Maryam Kohansal; Ali Taghinezhad
Abstract
Dairy products are susceptible to contamination by various microorganisms, including fungal species, throughout the production, distribution, and storage phases, primarily due to non-compliance with hygienic standards. Notably, fungal microorganisms exhibit resilience to acidic and low-temperature environments, ...
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Dairy products are susceptible to contamination by various microorganisms, including fungal species, throughout the production, distribution, and storage phases, primarily due to non-compliance with hygienic standards. Notably, fungal microorganisms exhibit resilience to acidic and low-temperature environments, enabling their proliferation in diverse food matrices. The majority of spoilage is attributed to aflatoxin-producing Aspergillus species, such as A. flavus, A. parasiticus, A. nomius, A. arizus, A. ariza, A. acreosus, and Penicillium puberculum. Among these, Aflatoxin B1 (AFB1) poses a significant threat due to its carcinogenic potential. Conventional methods for aflatoxin removal, including physical and chemical approaches, are often costly, potentially unsafe, and detrimental to food quality. Lactic acid bacteria (LAB), encompassing genera such as Lactobacillus, Leuconostoc, Pedicoccus, and Streptococcus, offer a promising alternative for aflatoxin removal.
