Abstract
Erythropoietin (EPO) is one of the most important hormones in the human body that plays a critical role in preventing cellular damage caused by hypoxia. Due to its effective pharmaceutical performance, recombinant human EPO (rhEPO) is often produced by Chinese Hamster Ovary (CHO) cells as host cells ...
Read More
Erythropoietin (EPO) is one of the most important hormones in the human body that plays a critical role in preventing cellular damage caused by hypoxia. Due to its effective pharmaceutical performance, recombinant human EPO (rhEPO) is often produced by Chinese Hamster Ovary (CHO) cells as host cells through recombinant DNA technology on an industrial scale. In this study, the reported genome-scale metabolic network of CHO cells was upgraded to integrate EPO production pathways using the INIT algorithm in the RAVEN Toolbox. After quality analysis for the reconstructed model, performance of the model was examined under two different culture conditions provided within the literature. Such analyses were implemented through Flux Balance Analysis and Multi-objective Analysis techniques and the results highlighted the effectiveness of these culture conditions. To enhance the efficiency of rhEPO production, analysis of essential genes and reactions, sensitivity of essential amino acid supplementation and flexibility of amino acid uptakes was also performed through a series of standard in silico techniques in constraint-based analysis.
Medicinal sciences
Khairun Nisa; Dewi Nur Fiana; Liana Sidharti
Abstract
This study aimed to determine the effect of Ambon banana fruit consumption on plasma MDA levels and other cardiometabolic indicators in adult men with central obesity. A total of 30 volunteers were involved. The volunteers ask to take 250 grams of Ambon banana for breakfast for 28 days. The volunteers ...
Read More
This study aimed to determine the effect of Ambon banana fruit consumption on plasma MDA levels and other cardiometabolic indicators in adult men with central obesity. A total of 30 volunteers were involved. The volunteers ask to take 250 grams of Ambon banana for breakfast for 28 days. The volunteers were examined for their plasma MDA levels and cardiometabolic indicators before and after treatment. The results showed that consuming Ambon banana fruit at breakfast for 28 days significantly reduced body weight, BMI, waist circumference, and systolic and diastolic blood pressure (p<0.05). Ambon banana intake has also been shown to reduce plasma MDA levels after treatment significantly (p<0.05), reaching 11.42% compared to MDA levels before treatment. Conclusion: Consuming Ambon banana at breakfast for 28 days improved cardiometabolic indicators and reduced plasma MDA levels in men with central obesity.
Biological sciences
Arsalan Jalili; Nasser Aghdami; Abbas Hajifathali; Maryam Sayahinouri
Abstract
Background: There are various mechanisms are being developed to expand Virus specific T lymphocytes. In this study, the use of anti-CD3/CD28 magnetic microbeads and IL-15 in the culture of T lymphocytes in vitro has a significant role in the proliferation and cytotoxicity of these cells.
Materials and ...
Read More
Background: There are various mechanisms are being developed to expand Virus specific T lymphocytes. In this study, the use of anti-CD3/CD28 magnetic microbeads and IL-15 in the culture of T lymphocytes in vitro has a significant role in the proliferation and cytotoxicity of these cells.
Materials and Methods: Peripheral blood mononuclear cells of 6 HCMV positive volunteers were harvested, then the pure population of lymphocytes were isolated. HCMV-Specific T Cells were expanded in 4 groups consisting of IL-2, IL-15 and anti-CD3/CD28 microbeads. Cells were assessed on the 14th day.
Results: A significant effect of IL-15 along with anti-CD3/CD28 magnetic microbeads on the proliferation rate of HCMV-specific T lymphocytes, and also increase the cytotoxicity and the secretion of gamma interferon.
Conclusion: The use of IL-15 along with anti-CD3/CD28 beads shows effective results for virus specific T cell proliferation.
Medicinal sciences
Ria Andreinie; Jeanne Adiwinata Adiwinata Pawitan
Abstract
Treatment of cartilage damage is not satisfactory mainly due to cartilage nature that is avascular, so that its nutrition only depends on the diffusion process, which does not support regeneration. One of the new approaches for regeneration of injured cartilage that have a future is tissue engineering. ...
Read More
Treatment of cartilage damage is not satisfactory mainly due to cartilage nature that is avascular, so that its nutrition only depends on the diffusion process, which does not support regeneration. One of the new approaches for regeneration of injured cartilage that have a future is tissue engineering. So far, there have been many clinical applications of tissue engineering in knee cartilage defects with varying results. However, it is not known, which method provides the best result. Therefore, this review highlighted the clinical applications of various tissue engineering methods in knee cartilage defects and compared the various procedures. Our result showed that the best healing results was tissue engineering using GelrinC, and the method that had the lowest side effects was tissue engineering using hydrogel-based autologous chondrocyte transplantation.
Pharmaceutical sciences
Foroogh Mirzania; Iraj Salimikia; Javad Ghasemian-Yadegari; Javid Aalaei; Mohammad Amin Ghobadi; Majid Taheri Demneh; Alireza Nazarzadeh
Abstract
In this research the protective effects of the methanolic extract of S. multicaulis was evaluated in renal ischaemia-reperfusion injuries in rats. 42 male rats were divided into 6 groups. In the treatment groups 1-3, before causing ischemia in the kidneys, rats received 50, 100, and 150 mg/kg/day doses ...
Read More
In this research the protective effects of the methanolic extract of S. multicaulis was evaluated in renal ischaemia-reperfusion injuries in rats. 42 male rats were divided into 6 groups. In the treatment groups 1-3, before causing ischemia in the kidneys, rats received 50, 100, and 150 mg/kg/day doses of the extract orally for 20 days, and in the fourth group, 20-days pretreatment with rosmarinic acid solution 0.125 mg/kg/day was administered intraperitoneally in DMSO and normal saline, and then ischaemia was created. In the evaluation of urea and creatinine factor, rosmarinic acid and extract dose of 150 mg/kg/day had a significant effect in reducing these two factors. It is estimated that doses of 100 and 150 mg/kg/day of methanol extract of S. multicaulis are the most effective and useful doses of this plant for renal ischemia reperfusion.
Medicinal sciences
Reza Nafisi Moghadam; Mojtaba Jokar; Shima sadat Dehghanpour farashah; Alirezaa Zolfagharii
Abstract
Breast cancer is the most malignancy in women. The aim of this study was to evaluate determining the characteristics of cerebral metastasis from breast cancer. This cross sectional study was conducted on patients with cerebral metastasis from breast cancer in Shahid Sadoughi hospital from 2014 to 2020 ...
Read More
Breast cancer is the most malignancy in women. The aim of this study was to evaluate determining the characteristics of cerebral metastasis from breast cancer. This cross sectional study was conducted on patients with cerebral metastasis from breast cancer in Shahid Sadoughi hospital from 2014 to 2020 and was conducted on 37 patients with mean age 50.83± 14.58 y/o. Negative estrogen receptor/ progesterone receptor and Ki67 > 50 was seen in 54.1%, 62.2 %, and 10.8 % of patients, respectively. The most common cerebral tumor was brain with frequency of 63.6 %. There was no significant difference between age range less and more than 50 years regarding cerebral metastasis and location of cerebral tumors (p-value >0.05). The most location of cerebral metastasis was brain. Furthermore, cerebral metastasis and location of cerebral tumors were not influenced by age range of patients.
