ORIGINAL_ARTICLE
Early detection of melanoma with synchrotron radiation X-ray fiber diffraction analysis
Skin is the largest organ in our body protecting us from ultraviolet radiation and xenobiotics. The function of keratinocytes is to form the impermeable barrier for the skin and rest of the body, whereas melanocytes provide the pigment for the keratinizing skin layers to protect the integrity of keratinocytes, basal cells, immune cells and many other dermo-epidermal structures. Pigmented nevi are considered to be precursor lesions for malignant melanoma of the skin. They harbor genetic alterations such as oncogene BRAF.
https://www.healthbiotechpharm.org/article_133864_f8d40d7f94559a759521b53772a25b1e.pdf
2017-09-01
1
4
10.22034/HBB.2017.22
Melanoma
Synchrotron radiation
X-ray fiber diffraction analysis
ORIGINAL_ARTICLE
Chemical aspects of antigen nano carriers for vaccine delivery
Scientists could take many approaches to design and classify vaccines against a microbe. These choices could be typically based on fundamental information about antigen carriers of vaccines, how to produce them synthetically and which chemical reactions involved for attaching antigen to carriers. This review briefly describes design and development of novel vaccine carriers.
https://www.healthbiotechpharm.org/article_133866_96564ea9d586fdeaf9c1b94f05d312a4.pdf
2017-09-01
5
24
10.22034/HBB.2017.08
Vaccine Delivery
nano carriers
Liposome
Dendrimer
cellular immune response
ORIGINAL_ARTICLE
Optimization of cyclotide like peptide extraction methods and characterization of these peptides from Viola tricolor
Cyclotides are antimicrobial peptides and play significant role as bio preservative in the food industry. Since foodborne diseases are a universal issue, in the present study the different methods for partial purification of cyclotide like peptides from Viola tricolor were compared and the antimicrobial activities of these peptides were investigated on Staphylococcus aureus Escherichia coli, which are important in foodborne diseases. Cyclotide like peptides from the aerial parts of the plant were extracted and it was partially purified with three methods including reverse phase chromatography, two phase system with reverse phase chromatography and ammonium sulfate precipitation. The samples were analyzed by SDS-PAGE, Tricine–PAGE, and reverse phase HPLC. It was found that the first method has lower MIC (Minimum Inhibitory Concentration) and is the best method.
https://www.healthbiotechpharm.org/article_133867_903648f2189136895d4f139cdbf0c702.pdf
2017-09-01
25
38
10.22034/HBB.2017.09
Cyclotides
Peptides
Viola tricolor
ORIGINAL_ARTICLE
Optimization of green synthesis of ZnO nanoparticles by Dittrichia graveolens (L.) aqueous extract
In this work, we reported synthesis of ZnO nanoparticles (ZnONPs) by green procedure. A simple and effective synthesis of ZnONPs was performed by Dittrichia graveolens aqueous extract. The effect of three parameters including pH of the Zinc solution (4.0, 6.0 and 8.0), time (40, 60 and 120 min) and extract ratio (25 and 75 %) were studied and optimized using Response Surface Methodology (RSM). The ZnONPs were characterized by UV–Vis, FTIR and FESEM methods. The size of particles were around 100 nm. This new eco-friendly synthesis of ZnONPs is a convenient technique for large scale commercial manufacture of ZnONPs.
https://www.healthbiotechpharm.org/article_133868_9f596cffc5782ffadfff352fa4a26aae.pdf
2017-09-01
39
49
10.22034/HBB.2017.10
Green synthesis
Dittrichia graveolens (L.)
Response surface methodology (RSM)
Zinc oxide nanoparticles
UV-Vis
FESEM
ORIGINAL_ARTICLE
Association scrutiny between the miRNA 148a/152 polymorphisms and risk of breast cancer in Isfahan population
Breast cancer is 26% of diagnosed cancer in 2008 and it is second cause of cancer related deaths in women. This type of cancer is a complex genetic disease, because microRNAs (miRNAs) are expressional regulators of genes, genetic variations such as single nucleotide polymorphisms in their genes. Therefore, this study was conducted to evaluate the association between single nucleotide polymorphisms (SNPs) in miRNA 148/152 genes and breast cancer risk in Isfahan population. This case control study was performed on 200 samples containing 100 controls and 100 cases. Polymorphism genotyping was performed using polymerase chain reaction restriction fragment length polymorphism (PCR–RFLP) and tetra primer amplification refractory mutation system (T-ARMS-PCR).Our observations indicated no significant association between rs185641358 and rs12940701 in miRNAs and breast cancer risk because their p-values are 0.08 and 0.19, respectively. No association between miRNA148/152 genes polymorphisms and risk of breast cancer was found.
https://www.healthbiotechpharm.org/article_133870_f2c3240ec2c17357c33abe78cd7a6804.pdf
2017-09-01
50
60
10.22034/HBB.2017.11
breast cancer
miRNA148a
miRNA152
SNP
ORIGINAL_ARTICLE
Association study of leptin and leptin receptor gene polymorphisms with diabetes type 2 and obesity
The adiposity hormone, leptin, plays an important role in the control of glucose metabolism by its action in the brain. The effects of leptin are reducing body adiposity, food intake and improving insulin sensitivity in peripheral tissue by indirect mechanism. This study was performed to investigate the prevalence and association A19G and K109R polymorphisms in leptin (LEP) and leptin receptor genes (LEPR) with diabetes and obesity in Yazd, Iran. In this case control study, the case groups were 100 obese people with type 2 diabetes mellitus and the control groups were 100 healthy people. Genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The allele frequency for A and G alleles in LEP gene (A19G) were 0.275 and 0.725, respectively. Also K and R alleles in LEPR gene (K109R) were 0.36 and 0.64, respectively. The genotype and allele frequencies were not significantly different for patient and control groups. HBA1C and leptin were high in patient group. The LEP and LEPR SNPs in this study may not be useful markers for obesity or diabetes in Iranian population but with attention to the past studies these SNPs may have synergistic effects on obesity and diabetes.
https://www.healthbiotechpharm.org/article_133872_320ca9fe8e38ef6e723d13b88509b5e8.pdf
2017-09-01
61
69
10.22034/HBB.2017.12
Polymorphism
Diabetes
Obesity
leptin
Leptin receptor
ORIGINAL_ARTICLE
The effect of Glycirrhiza globra on osteoarthritis, suppress TNF-a, IL-1b, Cox-2, iNOS gene expression and prostaglandin E2 and nitric oxide production in particular chondrocytes and monocyte/macrophages
In this study, we evaluated the effects of avocado soybean unsaponifiables (Glycirrhiza globra) on proinflammatory mediators in chondrocytes and monocyte/macrophage-like cells. Cells were activated with 20 ng/ml lipopolysaccharide (LPS) for 24 h and cell supernatants were analyzed for prostaglandin E2 (PGE2) and nitrite content. One set of cells was activated for 1 h with LPS (20 ng/ml) for both reverse-transcriptase polymerase chain reaction (RT-PCR) and real-time PCR analysis of tumor necrosis factor-alpha (TNF-a), interleukin-1-beta (IL-1b), cyclooxygenase-2 (Cox-2), and inducible nitric oxide synthase (iNOS) expression. One set of cells was activated for 24 h to analyze released PGE2 and nitrite levels in the cellular supernatant.Glycirrhiza globra reduced TNF-a, IL-1b, Cox-2, and iNOS. The suppression of Cox-2 and iNOS expression was significant reduced in PGE2 and nitrite, in the cellular supernatant. In this study, we demonstrate the effects of Glycirrhiza globra on the isolated bovine chondrocyte and the human monocyte/macrophage-like cells. These observations provide a scientific comprehensive map to reduce the pain of osteoarthritis.
https://www.healthbiotechpharm.org/article_133875_4cfb86ba51e262dab6678a5c18f1fabf.pdf
2017-09-01
70
82
10.22034/HBB.2017.13
osteoarthritis
chondrocytes
TNF- α
IL-1B
COX-2
iNOS