%0 Journal Article %T One pot, green synthesis and biological evaluation of some isatin-based thiocarbohydrazones derivatives %J Health Biotechnology and Biopharma (HBB) %I Health Biotechnology and Biopharma %Z 2538-4414 %A Shahcheragh, Seyyed Mohammad %A Habibi, Azizollah %A Shirzad, Masoomeh %A Farahani, Yekta %A Sardari, Soroush %D 2023 %\ 03/01/2023 %V 6 %N 4 %P 81-97 %! One pot, green synthesis and biological evaluation of some isatin-based thiocarbohydrazones derivatives %K Hiocarbohydrazone %K Deep eutectic solvent %K one pot and three-component condensation reaction %R 10.22034/HBB.2023.07 %X In the present study, a one-pot, green and three-component route for synthesis of Thiocarbohydrazone (TCS) derivatives is described. This reaction was performed in choline chloride/urea (1:2) as Deep Eutectic Solvent (DES). The structures were characterized by FTIR, 1H NMR, 13C NMR spectra and elemental analysis. The derivatives were evaluated for their anti-mycobacterial activity against Mycobacterium bovis BCG, and the results revealed that among the synthesized compounds (1a-1h, 2a-2h, 3a-3h), 3b, 3c and 1b exhibited the highest activity with MIC value of 7.81 and 11.71 μg/mL. Additionally, the target compounds were evaluated for their anti-microbial activity against E.coli and Candida Albicans and the result indicated that in contrast to the low antifungal activity, the produced derivatives with electron-withdrawing substitution at the ortho, meta and para positions of benzyl ring includes 1d, 2b and 3d-3g demonstrated remarkable anti-micobacterial activities. %U https://www.healthbiotechpharm.org/article_166945_fe9a9bab6851efc292171c25d306dd3e.pdf