Abstract
OmpA and OmpK36 from K. pneumoniae as the antigens and FliC from Salmonella typhimurium as the adjuvant were applied to construct a multi-epitope vaccine. B-cell, T-cell, and IL-17-epitopes were identified and a construct was modeled. Molecular docking was performed to assess the interaction between ...
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OmpA and OmpK36 from K. pneumoniae as the antigens and FliC from Salmonella typhimurium as the adjuvant were applied to construct a multi-epitope vaccine. B-cell, T-cell, and IL-17-epitopes were identified and a construct was modeled. Molecular docking was performed to assess the interaction between chimeric FliC and TLR-5. Downstream analysis including antigenicity, allergenicity, IFN-γ and IL-4 epitopes prediction was done. This construction covers both B-cell and T-cell epitopes as well as IFN-γ and IL-4 epitopes, but no IL-17 epitope was detected. We used two other known epitopes (IEDB epitope ID 43662 and epitope ID 57417) that induce the IL-17 release. According to the result, the multi-epitope protein is probable antigen and not an allergen. This construction was stable, hydrophilic, and has no transmembrane helixes. The computer-aided analysis imply that this protein is an acceptable candidate for immunization against K. pneumoniae.
Abstract
The aim of this study was to evaluate the resistance pattern and susceptibility of some antibiotics to K. pneumoniae isolated from urinary tract infections. In this descriptive cross-sectional study, 165 K. pneumoniae strains were collected from Tabriz hospitals during six months and were identified ...
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The aim of this study was to evaluate the resistance pattern and susceptibility of some antibiotics to K. pneumoniae isolated from urinary tract infections. In this descriptive cross-sectional study, 165 K. pneumoniae strains were collected from Tabriz hospitals during six months and were identified by standard laboratory tests in a dedicated environment. Antibiotic resistance assay was performed by disk diffusion method. The highest resistance of K. pneumoniae isolates to tetracycline antibiotics (71.5 %), ciprofloxacin (56.9 %) and ceftriaxone (41.8 %) and the highest sensitivity to gentamicin (87.9 %), imipenem (85.5 %) and Cefepime (81.8 %) obtained. With increasing prevalence of antibiotic resistance, early detection of antibiotic resistant strain is necessary to select appropriate treatment and prevent spread of antibiotic resistance.
