Abstract
Cathelicidin derived from human leukocytes is a 171amino acid protein that demonstrated to have antimicrobial activity. Synthesis of Antimicrobial Peptides (AMPs) are known to be expensive. In this study, after extraction of human cathelicidin sequence from NCBI, physicochemical characteristics such ...
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Cathelicidin derived from human leukocytes is a 171amino acid protein that demonstrated to have antimicrobial activity. Synthesis of Antimicrobial Peptides (AMPs) are known to be expensive. In this study, after extraction of human cathelicidin sequence from NCBI, physicochemical characteristics such as length, amino acids composition, hydrophobicity and net charge were determined. Several truncated peptides were selected and probability of antimicrobial activity was predicted by Antimicrobial Peptide Prediction software. Purity and molecular weight of the peptides were confirmed by HPLC and mass spectrometry. Eventually, Minimum Inhibitory Concentration (MIC) of these peptides against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were examined by micro-dilution method. Two peptides were synthesized as a result of bioinformatics method. One of the two prepared peptides with 13 amino acids length showed good activity against the bacteria. MIC results of this peptide were 15.6 μg/ml (against E. coli), 31.2 μg/ml (against P. aeruginosa) and 31.2 μg/ml (against S. aureus). Another peptide, Cth14, showed 125 μg/ml against E. coli. IC50 of Cth13 and Cth14 were 959 μg/ml and 335 μg/ml, respectively.
Abstract
In this study, we have shown that biologically synthesized Silver Nanoparticles (Ag-NPs) can effectively inhibit the growth of a clinical isolate of the Acinetobacter spp in vivo. The bacterial strain was isolated from a hospitalized male burnt patient in Sulaymaniyah, Iraq. Initially, Minimum Inhibitory ...
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In this study, we have shown that biologically synthesized Silver Nanoparticles (Ag-NPs) can effectively inhibit the growth of a clinical isolate of the Acinetobacter spp in vivo. The bacterial strain was isolated from a hospitalized male burnt patient in Sulaymaniyah, Iraq. Initially, Minimum Inhibitory Concentration (MIC) of ampicillin, kanamycin, tetracycline, and azithromycine were determined for the strain using broth microdilution method. The bacterial strain was found to have the highest resistant against azithromycin (400 μg/ml), whereas the MIC for kanamycin, ampicillin and tetracycline were at 200 μg/ml, 100 μg/ml, and 100 μg/ml, respectively. Additionally, Ag-NPs were prepared using the aqueous extract of chamomile (Matricaria chamomilla) flowers. Scanning Electron Microscopy (SEM) image revealed the particles have spherical and irregular flower-like shapes with an average size of 18 ±3 nm based on the X-Ray Diffraction (XRD) spectrum data. Interestingly, the biosynthesized Ag-NPs shown to actively inhibit the growth of the bacterial strain with MIC at 50 μg/ml.
Abstract
Phytochemical investigation of the aerial parts of Artemisia kopetdaghensis led to isolation and characterization of 5 compounds including 9-Oxo-tournefortiolide (1), 2,6,10-Trimethyl-2,5-epidioxy-7,10-epoxydodeca-3,11-dien-5-acetoxy (2), 6-Demethoxy-5,7-dihydroxy-4'-O-methylcapillarisin (3), Eupatilin ...
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Phytochemical investigation of the aerial parts of Artemisia kopetdaghensis led to isolation and characterization of 5 compounds including 9-Oxo-tournefortiolide (1), 2,6,10-Trimethyl-2,5-epidioxy-7,10-epoxydodeca-3,11-dien-5-acetoxy (2), 6-Demethoxy-5,7-dihydroxy-4'-O-methylcapillarisin (3), Eupatilin (4) and 5, 7, 5'-trihydroxy-6, 2´, 4´-trimethoxyflavone (5). The chemical structures of these compounds were identified by spectroscopic analysis. The antioxidant activities of the pure compounds were evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays and also the antimicrobial capacity were tested against gram positive and gram negative bacteria and fungi using minimum inhibitory concentration (MIC) method.
