Abstract
The present study is created to determine the effect of rs3783605 and its interaction with IL-17A, IL-17E and TNF-α cytokines on the action of VCAM-1 gene promoter in human umbilical vein endothelial cells (HUVEC). Recent research shows that IL-17A and TNF-α cytokines have main effect on ...
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The present study is created to determine the effect of rs3783605 and its interaction with IL-17A, IL-17E and TNF-α cytokines on the action of VCAM-1 gene promoter in human umbilical vein endothelial cells (HUVEC). Recent research shows that IL-17A and TNF-α cytokines have main effect on VCAM-1 expression, and IL-17E as a proinflammatory ligand for IL-17 receptor homolog IL-17Rh1 might also partake in this process. Two vectors with altered rs3783605 alleles are fabricated to express the GFP. Unlike the other two cytokines, HUVECs stimulated with TNF-α alone show an increase of expression. The stimulation with IL-17A in addition to TNF-α show an increase of expression in G allele vector, while the stimulation with IL-17E in addition to TNF-α led to an increased expression in the cells containing an allele. The stimulation by IL-17A in addition to IL-17E led to decrease of expression in the cells containing G allele and stimulation with three cytokines simultaneously showed a decrease of expression in G allele vector.
Abstract
Breast cancer is 26% of diagnosed cancer in 2008 and it is second cause of cancer related deaths in women. This type of cancer is a complex genetic disease, because microRNAs (miRNAs) are expressional regulators of genes, genetic variations such as single nucleotide polymorphisms in their genes. Therefore, ...
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Breast cancer is 26% of diagnosed cancer in 2008 and it is second cause of cancer related deaths in women. This type of cancer is a complex genetic disease, because microRNAs (miRNAs) are expressional regulators of genes, genetic variations such as single nucleotide polymorphisms in their genes. Therefore, this study was conducted to evaluate the association between single nucleotide polymorphisms (SNPs) in miRNA 148/152 genes and breast cancer risk in Isfahan population. This case control study was performed on 200 samples containing 100 controls and 100 cases. Polymorphism genotyping was performed using polymerase chain reaction restriction fragment length polymorphism (PCR–RFLP) and tetra primer amplification refractory mutation system (T-ARMS-PCR).Our observations indicated no significant association between rs185641358 and rs12940701 in miRNAs and breast cancer risk because their p-values are 0.08 and 0.19, respectively. No association between miRNA148/152 genes polymorphisms and risk of breast cancer was found.