Biological sciences
Arsalan Jalili; Nasser Aghdami; Abbas Hajifathali; Maryam Sayahinouri
Abstract
In this study, the use of anti-CD3/CD28 magnetic microbeads and IL-15 in the culture of T lymphocytes in vitro had a significant role in the proliferation and cytotoxicity of these cells. Peripheral blood mononuclear cells of 6 (Cytomegalovirus) HCMV positive volunteers were harvested, then the pure ...
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In this study, the use of anti-CD3/CD28 magnetic microbeads and IL-15 in the culture of T lymphocytes in vitro had a significant role in the proliferation and cytotoxicity of these cells. Peripheral blood mononuclear cells of 6 (Cytomegalovirus) HCMV positive volunteers were harvested, then the pure population of lymphocytes were isolated. HCMV-Specific T Cells were expanded in 4 groups consisting of IL-2, IL-15 and anti-CD3/CD28 microbeads. A significant effect of IL-15 along with anti-CD3/CD28 magnetic microbeads on the proliferation rate of HCMV-specific T lymphocytes was evaluated.