Medicinal sciences
Ria Andreinie; Jeanne Adiwinata Adiwinata Pawitan
Abstract
Treatment of cartilage damage is not satisfactory mainly due to cartilage nature that is avascular, so that its nutrition only depends on the diffusion process, which does not support regeneration. One of the new approaches for regeneration of injured cartilage that have a future is tissue engineering. ...
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Treatment of cartilage damage is not satisfactory mainly due to cartilage nature that is avascular, so that its nutrition only depends on the diffusion process, which does not support regeneration. One of the new approaches for regeneration of injured cartilage that have a future is tissue engineering. There have been many clinical applications of tissue engineering in knee cartilage defects with varying results that it is not known, which method provides the best result. Therefore, this review highlighted the clinical applications of various tissue engineering methods in knee cartilage defects and did comparison of the various procedures. Our result showed that the best healing results was tissue engineering using GelrinC, and the method that had the lowest side effects was tissue engineering using hydrogel-based autologous chondrocyte transplantation.
Abstract
Due to the side effects of current therapies for osteoarthritis one of the alternative medicine is using herbal medicine such as Nigella sativa L. We examined that alcoholic extract of Nigella sativa (AENS) has an anti-inflammatory activity. Cells were activated with 100 ng/ml lipopolysaccharide for ...
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Due to the side effects of current therapies for osteoarthritis one of the alternative medicine is using herbal medicine such as Nigella sativa L. We examined that alcoholic extract of Nigella sativa (AENS) has an anti-inflammatory activity. Cells were activated with 100 ng/ml lipopolysaccharide for 24 h and cell supernatants were analyzed for PGE2 and nitrite content. One set of cells was activated for 1 h with LPS (100 ng/ml) for both reverse-transcriptase PCR and real-time PCR analysis of TNF-a, IL-1b , COX-2, and iNOS expression. AENS also reduced TNF-α and IL-1b expression in LPS-activated THP-1 cells. The present study demonstrates that the anti-inflammatory activity of AENS is not restricted to synoviocytes, but also affects monocyte macrophage-like cells that serve as a prototype for macrophages in the synovial membrane. These observations provide a scientific rationale for the pain-reducing and anti-inflammatory effects of AENS observed in osteoarthritis patients.
Abstract
In this study, we evaluated the effects of avocado soybean unsaponifiables (Glycirrhiza globra) on proinflammatory mediators in chondrocytes and monocyte/macrophage-like cells. Cells were activated with 20 ng/ml lipopolysaccharide (LPS) for 24 h and cell supernatants were analyzed for prostaglandin E2 ...
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In this study, we evaluated the effects of avocado soybean unsaponifiables (Glycirrhiza globra) on proinflammatory mediators in chondrocytes and monocyte/macrophage-like cells. Cells were activated with 20 ng/ml lipopolysaccharide (LPS) for 24 h and cell supernatants were analyzed for prostaglandin E2 (PGE2) and nitrite content. One set of cells was activated for 1 h with LPS (20 ng/ml) for both reverse-transcriptase polymerase chain reaction (RT-PCR) and real-time PCR analysis of tumor necrosis factor-alpha (TNF-a), interleukin-1-beta (IL-1b), cyclooxygenase-2 (Cox-2), and inducible nitric oxide synthase (iNOS) expression. One set of cells was activated for 24 h to analyze released PGE2 and nitrite levels in the cellular supernatant.Glycirrhiza globra reduced TNF-a, IL-1b, Cox-2, and iNOS. The suppression of Cox-2 and iNOS expression was significant reduced in PGE2 and nitrite, in the cellular supernatant. In this study, we demonstrate the effects of Glycirrhiza globra on the isolated bovine chondrocyte and the human monocyte/macrophage-like cells. These observations provide a scientific comprehensive map to reduce the pain of osteoarthritis.
