Abstract
The aim of this study was investigation of blaCTX-M, blaSHV and blaTEM ESBLs among Escherichia coli (E. coli) isolates from meat products. Fifty sheep meat samples were prepared from some slaughter houses in Tehran, Iran. The isolates were also confirmed by conventional biochemical tests. The antibiotic ...
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The aim of this study was investigation of blaCTX-M, blaSHV and blaTEM ESBLs among Escherichia coli (E. coli) isolates from meat products. Fifty sheep meat samples were prepared from some slaughter houses in Tehran, Iran. The isolates were also confirmed by conventional biochemical tests. The antibiotic susceptibility of isolates was performed by disc diffusion method according to the clinical and laboratory standards institute (CLSI) 2016 guidelines. The polymerase chain reaction (PCR) was performed for detection of blaCTXM, blaSHV and blaTEM genes. Among 50 meat samples, 30 E. coli isolates were identified. The resistance rate to co-amoxiclav (86 %), doxycycline (10 %), tetracycline (6.7 %), cephamandole (10 %), cefazolin (6.7 %) and ceftazidime (6.7 %) were determined. The PCR screening clarified that all isolates were positive for blaCTXM (100 %) and 23.3 % were positive for blaTEM, but none of them amplified the blaSHV gene. Among E. coli isolates from meat, the antibiotic resistance rate was low, except for co-amoxiclav. High rate of blaCTX-M production among E. coli from meat is concerning owing to encoding resistance to B-lactam antibiotics and spread to human population.
