Helsy Junaidi; Jeanne Adiwinata Pawitan
Abstract
The development of tissue engineering provides various opportunities to vascular tissue engineering. Scaffold plays an essential role in vascular tissue engineering. The selection of biomaterials used as scaffolds will determine the success of vascular tissue engineering. The structure of vascular system, ...
Read More
The development of tissue engineering provides various opportunities to vascular tissue engineering. Scaffold plays an essential role in vascular tissue engineering. The selection of biomaterials used as scaffolds will determine the success of vascular tissue engineering. The structure of vascular system, which consists of three layers, is embedded in extracellular matrices that provide the mechanical properties of the system. Therefore, tissue engineering of a vascular structure needs various suitable biomaterials as scaffold that can support vascular system mechanical properties and function. Various materials were used for 3D printing and electro-spinning with good results, including collagen, gelatin, and alginate. Varying sizes of blood vessels require scaffolds with biomaterials that could adapt to their shape, size and approximate the mechanical properties of the blood vessels.
Abstract
In this study, angiogenesis inducers and inhibitors and some important drugs are introduced. Abnormal angiogenesis can occur if the balance between inhibitory and inducible factors disappears. Angiogenesis inducers include EGF, FGF2, TGF-β, VEGF, G-CSF, HGF, TGF-α, IL-8, TNF-α and angiogenesis ...
Read More
In this study, angiogenesis inducers and inhibitors and some important drugs are introduced. Abnormal angiogenesis can occur if the balance between inhibitory and inducible factors disappears. Angiogenesis inducers include EGF, FGF2, TGF-β, VEGF, G-CSF, HGF, TGF-α, IL-8, TNF-α and angiogenesis inhibitors such as endostatin, interleukin, prolactin, interferons, IL-1, IL-12, angiostatin, and IL-18. On the other hand, many drugs can affect these signaling pathways.
Abstract
Snake bite was known as one of the most important public health concerns world wild. Respiratory and cardiac problems and destruction of endothelium system occurs as a result of snake bite. Some proteins and peptides in snake venom have potential for drug discovery studies. According to the important ...
Read More
Snake bite was known as one of the most important public health concerns world wild. Respiratory and cardiac problems and destruction of endothelium system occurs as a result of snake bite. Some proteins and peptides in snake venom have potential for drug discovery studies. According to the important role of cell adhesion and angiogenesis in cancer development, identification of new therapeutics that targets this process seems indispensable. Evaluation of cytotoxicity, anti-adhesive and anti-angiogenic effects of Caspian Cobra snake (Naja oxiana) venom on human endothelial cell was the main aim of current study. Methods: Crude Cobra snake venom was fractionated by fast protein liquid chromatography (FPLC) using S-200 column. Purity of crude venom and each fraction was monitored by SDS-PAGE. Cytotoxicity of Caspian Cobra snake (Naja oxiana) crude venom and fractions on human endothelial cells was evaluated by MTT assay Adhesion and tube formation assay was performed to evaluate anti-adhesive and anti-angiogenic activities of snake venom. Results of FPLC revealed eight individual fractions. Cobra crude venom and fractions showed dose-dependent cytotoxic effect on human endothelial cell. Fraction 6 (IC50=5.7 μg/ml) and fraction 7 (IC50=5 μg/ml) showed higher cytotoxic effects on human endothelial cells. Therefore, further assays carried out with fraction 6 (F6) and 7 (F7) and results showed that both of fractions inhibited in vitro adhesion and tube formation of human endothelial cellsResults evaluated cytotoxicity, anti-adhesive and anti-angiogenic effects of Caspian Cobra snake (Naja oxiana) venom on human endothelial cell and represents promising tool for drug discovery and development.
