Abstract
Herbal medicine is one of the oldest human approaches to treating diseases and yarrow (Achillea wilhelmsii) one of the most famous medicinal plants. Yarrow essential oil has anti-inflammatory, anti-spasm, anti-scalp, hair growth stimulation and topical skin healing due to chamazulene, cineole and borneol. ...
Read More
Herbal medicine is one of the oldest human approaches to treating diseases and yarrow (Achillea wilhelmsii) one of the most famous medicinal plants. Yarrow essential oil has anti-inflammatory, anti-spasm, anti-scalp, hair growth stimulation and topical skin healing due to chamazulene, cineole and borneol. Staphylococcus aureus (S.aureus) and Escherichia coli (E.coli) are the most important bacteria causing nosocomial infections with multiple antibiotic resistances. Also, S.aureus strain is more sensitive than E.coli to extract of yarrow. Alpha-pinene, phenolic compounds and flavonoids are major constituents of yarrow. This review shows how methanol extract of yarrow prevented the growth of Gram-positive bacteria such as S.aureus and E.coli. So these extracts can be substituted for chemical drugs.
Abstract
Production of Extended Spectrum Beta Lactamase (ESBL) enzymes is one of the main reasons for drug resistance in Escherichia coli (E. coli) isolates. The aim of this study was to determine the ESBL producing E. coli isolates in the blood culture of patients in hospitals and medical centers in Tabriz, ...
Read More
Production of Extended Spectrum Beta Lactamase (ESBL) enzymes is one of the main reasons for drug resistance in Escherichia coli (E. coli) isolates. The aim of this study was to determine the ESBL producing E. coli isolates in the blood culture of patients in hospitals and medical centers in Tabriz, Iran. In this descriptive cross-sectional study, 200 isolates of E. coli from blood specimens in hospitals and medical centers were investigated from December 2018 to May 2019. The production of ESBLs was confirmed using the combined disc method and antimicrobial susceptibility test. Out of 200 isolates, 100 isolates (50 %) were able to produce ESBL. The highest antibiotic resistance of isolates (100 %) was to ceftriaxone, cefotaxime, cefixime and cefuroxime. The highest antimicrobial susceptibility was also observed to amikacin (12 %), meropenem (6 %) and imipenem (2 %), respectively. The results of this study showed the high resistance of E. coli isolates especially ESBL producing strains to different antibiotics. Given the relatively high prevalence of the ESBL producing E. coli strains and their resistance to common antibiotics, effective control strategies such as the restricted use of broad spectrum cephalosporins should be implemented.
Abstract
Increasing antibiotic resistance in strains of bacterial pathogens increase the attitude of people to find new compounds as an appropriate alternative to antibiotics. In this study, the antimicrobial effects of methanolic extract of Mentha pulegium against S. aureus and E. coli strains were investigated. ...
Read More
Increasing antibiotic resistance in strains of bacterial pathogens increase the attitude of people to find new compounds as an appropriate alternative to antibiotics. In this study, the antimicrobial effects of methanolic extract of Mentha pulegium against S. aureus and E. coli strains were investigated. After collecting the plants, essential oil of aerial parts of the plant was done by distillation with water through the clevenger apparatus. Gas chromatography-mass spectrometry (GC / MS) was used to identify the chemical composition of the essential oil. M. pulegium extract was prepared by soxhlet extractor method and their antimicrobial effects were investigated using agar well diffusion and dilution methods. The results of GC / MS analysis showed 22 compounds in M. pulegium plant. The results showed that M. pulegium methanolic extract prevents the growth of S. aureus and E. coli bacteria. Also, S. aureus showed a higher sensitivity to different concentrations of M. pulegium extract. Therefore, after supplementary studies, it could be used as a substitute the treatment of infections.
Abstract
The aim of this study was investigation of blaCTX-M, blaSHV and blaTEM ESBLs among Escherichia coli (E. coli) isolates from meat products. Fifty sheep meat samples were prepared from some slaughter houses in Tehran, Iran. The isolates were also confirmed by conventional biochemical tests. The antibiotic ...
Read More
The aim of this study was investigation of blaCTX-M, blaSHV and blaTEM ESBLs among Escherichia coli (E. coli) isolates from meat products. Fifty sheep meat samples were prepared from some slaughter houses in Tehran, Iran. The isolates were also confirmed by conventional biochemical tests. The antibiotic susceptibility of isolates was performed by disc diffusion method according to the clinical and laboratory standards institute (CLSI) 2016 guidelines. The polymerase chain reaction (PCR) was performed for detection of blaCTXM, blaSHV and blaTEM genes. Among 50 meat samples, 30 E. coli isolates were identified. The resistance rate to co-amoxiclav (86 %), doxycycline (10 %), tetracycline (6.7 %), cephamandole (10 %), cefazolin (6.7 %) and ceftazidime (6.7 %) were determined. The PCR screening clarified that all isolates were positive for blaCTXM (100 %) and 23.3 % were positive for blaTEM, but none of them amplified the blaSHV gene. Among E. coli isolates from meat, the antibiotic resistance rate was low, except for co-amoxiclav. High rate of blaCTX-M production among E. coli from meat is concerning owing to encoding resistance to B-lactam antibiotics and spread to human population.
Abstract
A novel series of complexes of the type [M (C18H17N2O2Cl)2], where M= Co(Ⅱ), Ni(Ⅱ), Cu(Ⅱ), Zn(Ⅱ) and Mn(Ⅱ) and C18H17N2O2Cl correspond to the bidentate ligands, were synthesized, and characterized by UV-Vis, FTIR, and Mass spectroscopies. The bidentate ligand was synthesized in a typical one-pot, ...
Read More
A novel series of complexes of the type [M (C18H17N2O2Cl)2], where M= Co(Ⅱ), Ni(Ⅱ), Cu(Ⅱ), Zn(Ⅱ) and Mn(Ⅱ) and C18H17N2O2Cl correspond to the bidentate ligands, were synthesized, and characterized by UV-Vis, FTIR, and Mass spectroscopies. The bidentate ligand was synthesized in a typical one-pot, three-component condensation of malononitrile, 4-chlorobenzaldehyde, and dimedone in the present of alum as catalyst. The synthesized ligand and its metal complexes were screened for in vitro antibacterial activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aurous) bacterial strains. The most antibacterial activity of the synthesized compounds belongs to cobalt and copper complexes. The minimal inhibitory concentrations (MICs) against E.Coli and S.aureus were 16 and 32 μg/ml, respectively. The results of these studies showed that the metal complexes have more antibacterial activities as compared with the non-complexes ligand.
