Biological sciences
Saghar Bakhtiari; Fatemeh Noorbakhsh; Sadaf Mohseni Fard; Raheleh Safaei javan
Abstract
The aim of this research was to investigate the effect of Zataria multiflora and silver nanoparticles on the biofilm formation ability of A. baumannii and the expression of genes involved in this process. Thirty isolates of A. baumannii bacteria were collected and studied from the Milad Hospital of Tehran. ...
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The aim of this research was to investigate the effect of Zataria multiflora and silver nanoparticles on the biofilm formation ability of A. baumannii and the expression of genes involved in this process. Thirty isolates of A. baumannii bacteria were collected and studied from the Milad Hospital of Tehran. The effects of Zataria multiflora essential oil and silver nanoparticles on the biofilm formation process were analyzed using the microtiter plate method. Also, the gene expressions of pil and aba after exposure to nanoparticles and essential oil were measured. Treatment the strains with Zataria multiflora essential oil at the MIC concentration, along with silver nanoparticles, reduced biofilm formation by the strains assessed and the expression of the genes noted. It seems that Z.multiflora essential oil and silver nanoparticles, can be used as candidate drugs in treatment or disinfection.
Abstract
Composition and chemical variation in the essential oil yield were studied among five wild growing populations of Dracocephalum Kotschyi Boiss. (Lamiaceae) from Iran. The hydrodistilled essential oils were isolated from aerial flowering parts of plant, analyzed by capillary GC-FID (Gas Chromatography ...
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Composition and chemical variation in the essential oil yield were studied among five wild growing populations of Dracocephalum Kotschyi Boiss. (Lamiaceae) from Iran. The hydrodistilled essential oils were isolated from aerial flowering parts of plant, analyzed by capillary GC-FID (Gas Chromatography Flame Ionization Detector) and GC-MS (Gas Chromatography Mass Spectrometry) were identified. Forty compounds were characterized represented 95.0 – 100 % of the total oil. Oxygenated monoterpenes constituted the main group of compounds (79.9-50.7 %) in the essential oils of all samples followed by monoterpene hydrocarbons (46.1-13.9 %). Perilla aldehyde (49.3 %), limonene (41.6 %), neral (20.9 %) and geranial (22.0 %) were found to be the main compounds. Such great variations in the components of the oils may be ascribed to existence of chemotypes and adaptation of the species to specific habitat. The data obtained contribute to broaden the inventory of wild Dracocephalum Kotschyi populations of Iran to plan programs for the selection of chemotypes with new and specific uses in food and drug industries.
Abstract
Increasing antibiotic resistance in strains of bacterial pathogens increase the attitude of people to find new compounds as an appropriate alternative to antibiotics. In this study, the antimicrobial effects of methanolic extract of Mentha pulegium against S. aureus and E. coli strains were investigated. ...
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Increasing antibiotic resistance in strains of bacterial pathogens increase the attitude of people to find new compounds as an appropriate alternative to antibiotics. In this study, the antimicrobial effects of methanolic extract of Mentha pulegium against S. aureus and E. coli strains were investigated. After collecting the plants, essential oil of aerial parts of the plant was done by distillation with water through the clevenger apparatus. Gas chromatography-mass spectrometry (GC / MS) was used to identify the chemical composition of the essential oil. M. pulegium extract was prepared by soxhlet extractor method and their antimicrobial effects were investigated using agar well diffusion and dilution methods. The results of GC / MS analysis showed 22 compounds in M. pulegium plant. The results showed that M. pulegium methanolic extract prevents the growth of S. aureus and E. coli bacteria. Also, S. aureus showed a higher sensitivity to different concentrations of M. pulegium extract. Therefore, after supplementary studies, it could be used as a substitute the treatment of infections.
Abstract
In this study, we identified the chemical composition and antimicrobial effects of different parts
of essential oils of Nepeta gloeocephala. Totally 65 volatile compounds from the essential oils in N. gloeocephala were obtained by means of hydrodistillation and its components were analyzed using GC ...
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In this study, we identified the chemical composition and antimicrobial effects of different parts
of essential oils of Nepeta gloeocephala. Totally 65 volatile compounds from the essential oils in N. gloeocephala were obtained by means of hydrodistillation and its components were analyzed using GC and GC-MS. These compounds are mainly oxygenated monoterpenes, monoterpene hydrocarbons and oxygenated sesquiterpenoids compound. The antimicrobial activity of leaf, flower, stem and root essential oils from N. gloeocephala were studied against seven gram-positive and gram-negative bacteria and two fungi by the disc diffusion method. The results of the bio assays showed an interesting antimicrobial activity, in which the gram-positive bacteria, Bacillus cereus and Staphylococcus aureus, were the most sensitive to the oils, as well the oils exhibited a remarkable antifungal activity against all the tested fungi.
