Biological sciences
Mahdi Zarei; Mohsen Soroush; Saeed Mohammadi
Abstract
Numerous studies have examined the effect of genetic variations in the Toll-Like Receptor 9 (TLR9) on the development of Systemic Lupus Erythematosus (SLE). An allele-specific PCR technique was employed to analyze the genetic variation in the study participants in a cohort of SLE and healthy controls ...
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Numerous studies have examined the effect of genetic variations in the Toll-Like Receptor 9 (TLR9) on the development of Systemic Lupus Erythematosus (SLE). An allele-specific PCR technique was employed to analyze the genetic variation in the study participants in a cohort of SLE and healthy controls from the Golestan region of northern Iran. Our study revealed no statistically significant variations in genotype and allele distribution between individuals with SLE and the control group. When analyzing the genotype frequency for rs352139, no significant association was observed between the GA genotype and AA genotype in relation to the risk of developing SLE, using the GG genotype as the reference. These findings indicated that the TLR9 rs352139 SNP could not play a significant role in determining susceptibility to SLE.
Biological sciences
Asifa Bibi; Nabeela Tariq; Hamida Ali; Zil-e-Huma Butt; Tasleem Kausar; Maqsood Maryam
Abstract
KEL1 (Kell or K) is highly immunogenic with limited distribution while KEL2 (Cellano or k) antigen is more prevalent. The antigenic diversity in the Kell gene is a result of Single Nucleotide Polymorphisms (SNPs). Blood samples of 160 males and females from each ethnic group with no age restrictions ...
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KEL1 (Kell or K) is highly immunogenic with limited distribution while KEL2 (Cellano or k) antigen is more prevalent. The antigenic diversity in the Kell gene is a result of Single Nucleotide Polymorphisms (SNPs). Blood samples of 160 males and females from each ethnic group with no age restrictions were subjected to Tetra-ARMS PCR to genotype the SNP (C578T) on exon 6 of chromosome 7. Genotype and allele frequencies were estimated in correlation with gender, age, and ethnicity with p-value less than 0.05. In general, antigen KEL2 k was more prevalent than antigen KEL1 k.
Abstract
Numerous genes with Single Nucleotide Polymorphisms (SNPs) are implicated in the Type 2 Diabetes Mellitus (T2DM) development. This work conducted as a case-control to assess the association of IFIH1 gene polymorphism (Ala946Thr) to the T2DM and hypertension among Iraqi patients. A total of 100 T2DM patients ...
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Numerous genes with Single Nucleotide Polymorphisms (SNPs) are implicated in the Type 2 Diabetes Mellitus (T2DM) development. This work conducted as a case-control to assess the association of IFIH1 gene polymorphism (Ala946Thr) to the T2DM and hypertension among Iraqi patients. A total of 100 T2DM patients were enrolled, 50 of them had hypertension. Moreover, 100 subjects were healthy that used as control group. All participants were genotyped using Real Time Polymerase Chain Reaction (RT-PCR). The G allele was more frequent in T2DM+ Hypertension (HTN) and T2DM without HTN (0.51 % and 0.52 %, respectively), while were less frequent (0.425 %) in the control group. The AA genotype was a significant risk factor for T2DM -HTN development. The GA genotype was also significantly (p <0.01 and OR: 5.8-8.3 associated, while the combination of the genotypes GA and AA depicted a significant P value<0.03 and OR of 4.42. The GA genotype was significantly related to the T2DM without hypertension.
Abstract
Genetic variations in Interleukin-10 (IL-10), an anti-inflammatory cytokine, has the potential to influence the development of Ischemic Stroke (IS). The current study aimed to examine IL-10 gene polymorphisms (rs1800896, rs1800871 and rs1800872) in association with IS. After genotyping, associational ...
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Genetic variations in Interleukin-10 (IL-10), an anti-inflammatory cytokine, has the potential to influence the development of Ischemic Stroke (IS). The current study aimed to examine IL-10 gene polymorphisms (rs1800896, rs1800871 and rs1800872) in association with IS. After genotyping, associational analyses showed a significant correlation of rs1800896 with the elevated risk of disease. Whereas for rs1800871, a decreased risk was observed and it might play a protective role. However, rs1800872 did not reveal an association with the predisposition to IS. Furthermore, all the haplotypes were significantly associated with the disease risk except GCA. The acquired Dʹ value from linkage disequilibrium showed the tendency of co-inheritance. Thus, the obtained results suggested that rs1800896 and rs1800871 have a significant relationship with IS.
Abstract
The adiposity hormone, leptin, plays an important role in the control of glucose metabolism by its action in the brain. The effects of leptin are reducing body adiposity, food intake and improving insulin sensitivity in peripheral tissue by indirect mechanism. This study was performed to investigate ...
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The adiposity hormone, leptin, plays an important role in the control of glucose metabolism by its action in the brain. The effects of leptin are reducing body adiposity, food intake and improving insulin sensitivity in peripheral tissue by indirect mechanism. This study was performed to investigate the prevalence and association A19G and K109R polymorphisms in leptin (LEP) and leptin receptor genes (LEPR) with diabetes and obesity in Yazd, Iran. In this case control study, the case groups were 100 obese people with type 2 diabetes mellitus and the control groups were 100 healthy people. Genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The allele frequency for A and G alleles in LEP gene (A19G) were 0.275 and 0.725, respectively. Also K and R alleles in LEPR gene (K109R) were 0.36 and 0.64, respectively. The genotype and allele frequencies were not significantly different for patient and control groups. HBA1C and leptin were high in patient group. The LEP and LEPR SNPs in this study may not be useful markers for obesity or diabetes in Iranian population but with attention to the past studies these SNPs may have synergistic effects on obesity and diabetes.
