Abstract
In this study, we have shown that biologically synthesized Silver Nanoparticles (Ag-NPs) can effectively inhibit the growth of a clinical isolate of the Acinetobacter spp in vivo. The bacterial strain was isolated from a hospitalized male burnt patient in Sulaymaniyah, Iraq. Initially, Minimum Inhibitory ...
Read More
In this study, we have shown that biologically synthesized Silver Nanoparticles (Ag-NPs) can effectively inhibit the growth of a clinical isolate of the Acinetobacter spp in vivo. The bacterial strain was isolated from a hospitalized male burnt patient in Sulaymaniyah, Iraq. Initially, Minimum Inhibitory Concentration (MIC) of ampicillin, kanamycin, tetracycline, and azithromycine were determined for the strain using broth microdilution method. The bacterial strain was found to have the highest resistant against azithromycin (400 μg/ml), whereas the MIC for kanamycin, ampicillin and tetracycline were at 200 μg/ml, 100 μg/ml, and 100 μg/ml, respectively. Additionally, Ag-NPs were prepared using the aqueous extract of chamomile (Matricaria chamomilla) flowers. Scanning Electron Microscopy (SEM) image revealed the particles have spherical and irregular flower-like shapes with an average size of 18 ±3 nm based on the X-Ray Diffraction (XRD) spectrum data. Interestingly, the biosynthesized Ag-NPs shown to actively inhibit the growth of the bacterial strain with MIC at 50 μg/ml.
Abstract
In recent years, application of medicinal plants increase in the treatment of infections rather than antimicrobial drugs. The aim of this study was to compare the effects of silver nanoparticles and alcoholic extract of Amaranthus retroflexus on some pathogenic bacteria. The alcoholic extract of this ...
Read More
In recent years, application of medicinal plants increase in the treatment of infections rather than antimicrobial drugs. The aim of this study was to compare the effects of silver nanoparticles and alcoholic extract of Amaranthus retroflexus on some pathogenic bacteria. The alcoholic extract of this plant was prepared and the effect of concentrations of 50 to 400 mg/ml of this extract and concentrations of 10 to 80 mg/ml of silver nanoparticles by well diffusion method on S. aureus, B. cereus, E coli and P aeruginosa were evaluated. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined on bacteria by dilution method. The results showed that the inhibition effect of Amaranthus retroflexus on gram-positive bacteria is more than gram negative bacteria while the inhibitory effect of silver nanoparticles on gram-negative bacteria is more than gram-positive bacteria. The results of this study showed that the alcoholic extract of Amaranthus retroflexus had an antibacterial properties that can be considered as an adjunct to further studies for in vivo conditions and replacement with common chemical agents in the treatment of infections.
