Biological sciences
Arsalan Jalili; Nasser Aghdami; Abbas Hajifathali; Maryam Sayahinouri
Abstract
Background: There are various mechanisms are being developed to expand Virus specific T lymphocytes. In this study, the use of anti-CD3/CD28 magnetic microbeads and IL-15 in the culture of T lymphocytes in vitro has a significant role in the proliferation and cytotoxicity of these cells.
Materials and ...
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Background: There are various mechanisms are being developed to expand Virus specific T lymphocytes. In this study, the use of anti-CD3/CD28 magnetic microbeads and IL-15 in the culture of T lymphocytes in vitro has a significant role in the proliferation and cytotoxicity of these cells.
Materials and Methods: Peripheral blood mononuclear cells of 6 HCMV positive volunteers were harvested, then the pure population of lymphocytes were isolated. HCMV-Specific T Cells were expanded in 4 groups consisting of IL-2, IL-15 and anti-CD3/CD28 microbeads. Cells were assessed on the 14th day.
Results: A significant effect of IL-15 along with anti-CD3/CD28 magnetic microbeads on the proliferation rate of HCMV-specific T lymphocytes, and also increase the cytotoxicity and the secretion of gamma interferon.
Conclusion: The use of IL-15 along with anti-CD3/CD28 beads shows effective results for virus specific T cell proliferation.
Biological sciences
Mohammad Ali Pahlevan Neshan; Payam Shahnazi Gerdehsang; Ahmad Bereimipour; Nazila Bostanshirin; Haniyeh Sadat Hosseininia; Kaykhosro Moridi; Amin Ebrahimi Sadrabadi; Arsalan Jalili
Abstract
Chronic inflammation in colorectal cancer can result in the progression of tumorigenesis. Inflammation in the colorectal part of the body can be affected by microbiota, as well as an individual diet. These two factors play a critical role in cancer types related to the colon and rectum. This inflammation ...
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Chronic inflammation in colorectal cancer can result in the progression of tumorigenesis. Inflammation in the colorectal part of the body can be affected by microbiota, as well as an individual diet. These two factors play a critical role in cancer types related to the colon and rectum. This inflammation could lose the protective epithelial layer of colorectal cancerous cells and, thus, translocation of healthy flora to inner layers. Pathogenic bacteria can also cause inflammation induction and tumorigenesis in the intestines. A change in the bacteria composition and some bacteria metabolites can also play a role in colorectal cancer. Dysbiosis can be affected by an individual diet. In this paper, the importance of diet and microbiota content on inflammation has been reviewed, and finally, in silico investigation confirmed their interplay.
