Samira Beyramy; Soroush Sardari
Abstract
Erythropoietin (EPO) is one of the most important hormones in the human body, due to its effective pharmaceutical performance, recombinant human EPO (rhEPO) is often produced by Chinese Hamster Ovary (CHO) cells as host cells through recombinant DNA technology on an industrial scale. In this study, the ...
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Erythropoietin (EPO) is one of the most important hormones in the human body, due to its effective pharmaceutical performance, recombinant human EPO (rhEPO) is often produced by Chinese Hamster Ovary (CHO) cells as host cells through recombinant DNA technology on an industrial scale. In this study, the reported genome-scale metabolic network of CHO cells was upgraded to integrate EPO production pathways using the INIT algorithm in the RAVEN Toolbox. After quality analysis for the reconstructed model, performance of the model was examined under two different culture conditions provided within the literature. Such analysis were implemented through Flux Balance Analysis (FBA) and Multi-objective Analysis techniques and the results highlighted the effectiveness of these culture conditions. To enhance the efficiency of rhEPO production, analysis of essential genes and reactions, sensitivity of essential amino acid supplementation and flexibility of amino acid uptakes was also performed through a series of standard in silico techniques in constraint-based analysis.
Seyyed Mohammad Shahcheragh; Azizollah Habibi; Masoomeh Shirzad; Yekta Farahani; Soroush Sardari
Abstract
In the present study, a one-pot, green and three-component route for synthesis of Thiocarbohydrazone (TCS) derivatives is described. This reaction was performed in choline chloride/urea (1:2) as Deep Eutectic Solvent (DES). The structures were characterized by FTIR, 1H NMR, 13C NMR spectra and elemental ...
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In the present study, a one-pot, green and three-component route for synthesis of Thiocarbohydrazone (TCS) derivatives is described. This reaction was performed in choline chloride/urea (1:2) as Deep Eutectic Solvent (DES). The structures were characterized by FTIR, 1H NMR, 13C NMR spectra and elemental analysis. The derivatives were evaluated for their anti-mycobacterial activity against Mycobacterium bovis BCG, and the results revealed that among the synthesized compounds (1a-1h, 2a-2h, 3a-3h), 3b, 3c and 1b exhibited the highest activity with MIC value of 7.81 and 11.71 μg/mL. Additionally, the target compounds were evaluated for their anti-microbial activity against E.coli and Candida Albicans and the result indicated that in contrast to the low antifungal activity, the produced derivatives with electron-withdrawing substitution at the ortho, meta and para positions of benzyl ring includes 1d, 2b and 3d-3g demonstrated remarkable anti-micobacterial activities.
Biological sciences
Seyedeh Mahsa Hosseini; Raheem Haddad; Soroush Sardari
Abstract
It has been found that natural microorganism have a siginificant role in the production and development of new medicines. The present study aimed to evaluat biotransformation of atropine, 4-hydroxy coumarin, 8-hydroxy quinoline, indole and industrially compound (penicillin G), arrive in the human body, ...
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It has been found that natural microorganism have a siginificant role in the production and development of new medicines. The present study aimed to evaluat biotransformation of atropine, 4-hydroxy coumarin, 8-hydroxy quinoline, indole and industrially compound (penicillin G), arrive in the human body, by some natural microorganism (Escherichia coli ATCC 25922, Bacillus subtilis ATCC 6051, Lactobacillus acidophilus ATCC 314, Mycobacterium Bovis (BCG), Saccharomyces cerevisiae ATCC 5052, and Candida albicans ATCC 10231), for the first time. The best values for the concentration and required times were evaluated for each metabolites in microbioal biotransformation process. The progress of the formation of the metabolites were monitored by Thin Layer Chromatography (TLC) method. Obtained results showed that only indole, atropine, and 4-hydroxy coumarin could be biotransformed by Candida albicans ATCC 10231, Escherichia coli ATCC 25922, and Saccharomyces cerevisiae ATCC 5052, respectively.
