Biological sciences
Saghar Bakhtiari; Fatemeh Noorbakhsh; Sadaf Mohseni Fard; Raheleh Safaei javan
Abstract
The aim of this research was to investigate the effect of Zataria multiflora and silver nanoparticles on the biofilm formation ability of A. baumannii and the expression of genes involved in this process. Thirty isolates of A. baumannii bacteria were collected and studied from the Milad Hospital of Tehran. ...
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The aim of this research was to investigate the effect of Zataria multiflora and silver nanoparticles on the biofilm formation ability of A. baumannii and the expression of genes involved in this process. Thirty isolates of A. baumannii bacteria were collected and studied from the Milad Hospital of Tehran. The effects of Zataria multiflora essential oil and silver nanoparticles on the biofilm formation process were analyzed using the microtiter plate method. Also, the gene expressions of pil and aba after exposure to nanoparticles and essential oil were measured. Treatment the strains with Zataria multiflora essential oil at the MIC concentration, along with silver nanoparticles, reduced biofilm formation by the strains assessed and the expression of the genes noted. It seems that Z.multiflora essential oil and silver nanoparticles, can be used as candidate drugs in treatment or disinfection.
Maryam Naderi Mezajin; Mahdi Fatemizadeh; Zahra Rostami; Pejvak Khaki; Masoomeh Shirzad; Fatemeh Noorbakhsh
Abstract
The aim of this study was to investigate the antibiotic resistance and identification of strains producing broad-spectrum beta-lactamases salmonella strains. The 70 different strains of salmonella were studied that gentamicin, ciprofloxacin, ofloxacin, imipenem, and enrofloxacin were susceptible. The ...
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The aim of this study was to investigate the antibiotic resistance and identification of strains producing broad-spectrum beta-lactamases salmonella strains. The 70 different strains of salmonella were studied that gentamicin, ciprofloxacin, ofloxacin, imipenem, and enrofloxacin were susceptible. The highest resistance to cephalexin (96 %), cefazolin (96 %), cephalothin (65 %), and 69 isolates (98 %), were resistant to more than one antibiotic. The results of the Polymerase Chain Reaction (PCR) were evaluated on ESBL producing isolates using specific primers; in order to investigate the presence of CTX-Mbla, SHVbla, TEMbla and OXAbla, so the presence of germ cell resistance genes in total indicated that 32 isolates (56 %), at least one of the above genes.